ASSESSMENT OF HYDROGEN PEROXIDE GENERATION IN NEUTROPHILS

以往的研究证实，基于双氢罗丹明(DHR)的流式细胞仪检测氧化爆发是评估慢性肉芽肿病(CGD)患者吞噬细胞的一种敏感方法。它也被用作筛查可能的X连锁CGD携带者的准确测试和疾病基因的初步筛查。对该方法进行了优化，对小于等于10,000个异常细胞中的一个正常细胞具有较高的灵敏度。一项追踪CGD患者输注异基因白细胞后的粒细胞的项目已经被证明在确定输注细胞存活和变态反应的持续时间方面是非常可靠的。这项研究的结果在美国血库协会年会上公布。CGD的第二阶段基因治疗继续针对X连锁CGD患者。DHR试验是一种关键方法，用于确定循环中是否存在基因校正的粒细胞，并监测治疗患者中基因校正的细胞的产生时间。

Previous work established that the flow cytometric dihydrorhodamine (DHR)-based assay for evaluation of oxidative burst is a sensitive method for evaluating phagocytes in patients with possible chronic granulomatous disease (CGD). It has also been useful as an accurate test for screening possible X-linked CGD carriers and a preliminary screen for the genotype of the disease. The method was optimized and is sensitive to one normal cell in less than or equal to 10,000 abnormal cells. A project was undertaken to track granulocytes post-transfusion in CGD patients following allogeneic leukocyte transfusions and this assay has proven to be very reliable in establishing duration of transfused cell survival and allsensitization. The results of this study were presented at the American Association of Blood Banks annual meeting. The second phase of gene therapy of CGD continues directed at patients with X-linked CGD. The DHR assay is being applied as a critical method for establishing the presence of gene-corrected granulocytes in the circulation and monitoring the duration of production of the gene-corrected cells in the treated patients