Assessment of Peripheral Blood Monocytes in Patients with Recurrent Mycobacterial

复发性分枝杆菌患者外周血单核细胞的评估

• 批准号: 6103714
• 负责人: thomas a fleisher
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6103714
• 关键词: CD antigens; CD40 molecule; Mycobacterium; bacterial disease; clinical research; cytokine receptors; human subject; immunodeficiency; immunopathology diagnosis; interferon gamma; monocyte; receptor expression

Monocytes are being characterized for the expression levels of CD40, CD80, CD86, CD120b, and the gamma interferon receptor alpha chain. These studies have identified two patients with gamma interferon receptor alpha chain deficiency, and the molecular level of this defect is being actively examined. In addition, one patient with interferon gamma receptor beta chain deficiency also has been characterized at the molecular level. As a consequence of these studies, an intracellular flow cytometric method for evaluating STAT protein phosphorylation has been developed and validated. This approach can be adapted for use for a wide variety of activation associated protein alterations. This line of investigation currently is being pursued.

单核细胞的特征是 CD40、CD80、CD86、CD120b 和 γ 的表达水平 干扰素受体α链。这些研究确定了两种 患有γ干扰素受体α链缺陷的患者，以及 目前正在积极研究这种缺陷的分子水平。在 此外，一名患有干扰素γ受体β链的患者 缺陷也已在分子水平上得到表征。作为一个 这些研究的结果是，细胞内流式细胞术 评价STAT蛋白磷酸化的方法 开发并验证。该方法可适用于 多种与激活相关的蛋白质改变。这条线 目前正在进行调查。