Assessment of Peripheral Blood Monocytes in Patients with Recurrent Mycobacterial

单核细胞的特征在于 CD 40、CD 80、CD 86、CD 120 b和γ-CD的表达水平 干扰素受体α链。这些研究确定了两个 γ干扰素受体α链缺陷患者，和 目前正在积极研究这一缺陷的分子水平。在 此外，1例干扰素γ受体β链 在分子水平上也对缺陷进行了表征。作为 这些研究的结果，细胞内流式细胞术 一种评价STAT蛋白磷酸化的方法， 开发和验证。这种方法可以适用于 各种各样的活化相关蛋白质改变。这条线 目前正在进行调查。

Monocytes are being characterized for the expression levels of CD40, CD80, CD86, CD120b, and the gamma interferon receptor alpha chain. These studies have identified two patients with gamma interferon receptor alpha chain deficiency, and the molecular level of this defect is being actively examined. In addition, one patient with interferon gamma receptor beta chain deficiency also has been characterized at the molecular level. As a consequence of these studies, an intracellular flow cytometric method for evaluating STAT protein phosphorylation has been developed and validated. This approach can be adapted for use for a wide variety of activation associated protein alterations. This line of investigation currently is being pursued.