NEIBANK: EST Analysis/Bioinformatics for Ocular Genomics

NEIBANK：眼部基因组学 EST 分析/生物信息学

• 批准号: 6227954
• 负责人: Graeme J Wistow
• 金额: --
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6227954
• 关键词: eye; genetic library; genetic registry /resource /referral center; human genetic material tag; information dissemination

The eye is a complex system of highly differentiated tissues of various developmental origins. Many genes essential for eye function are tissue-specific and many of those known are associated with genetic eye diseases. The majority of human expressed genes are known only through expressed sequence tags (ESTs). However, with the exception of retina, many eye tissues have been poorly represented in the cDNA libraries that have so far contributed to dbEST. To address this situation a project, NEIBANK, has been initiated to improve the EST coverage of the human eye. cDNA libraries for lens, ciliary body/iris and RPE/choroid have been constructed. 2000 clones from each un-normalized library have been sequenced, analyzed and clustered. Several novel genes have been identified, including Oculoglycan (an eye-specific LRR protein whose gene maps close to ARMD), LGS (an abundant lens specific transcript related to glutamine synthestase), Oculospanin (a novel tetraspan that maps to chr17) and others. Normalization and deeper sequence is under way. This resource will be of value for micro-arrays and for full- length cDNA collections. - EST, Bioinformatics, cDNA, genomics

眼睛是一个由不同发育来源的高度分化的组织组成的复杂系统。许多对眼睛功能至关重要的基因是组织特异性的，其中许多已知的基因与遗传性眼病有关。大多数人类表达的基因都是通过表达序列标签(EST)才知道的。然而，除了视网膜外，许多眼睛组织在迄今参与dBEST的cDNA文库中的表达水平很低。为了解决这种情况，已经启动了一个名为NEIBANK的项目，以提高EST对人眼的覆盖率。已构建了晶状体、睫状体/虹膜和RPE/脉络膜的cDNA文库。对每个非标准化文库中的2000个克隆进行了测序、分析和聚类。一些新的基因已经被发现，包括Oculoglycan(一种眼睛特异的LRR蛋白，其基因图谱接近ARMD)，Lgs(与谷氨酰胺合成酶相关的丰富的晶状体特异性转录本)，Oculospanin(一种新的定位于chr17的Tetraspan)等。正常化和更深层次的序列正在进行中。这一资源将对微阵列和全长cdna收集有价值。-EST、生物信息学、cDNA、基因组学