NEIBank: Est Analysis And Bioinformatics For Ocular Genomics

NEIBank：眼部基因组学的 Est 分析和生物信息学

• 批准号: 7594063
• 负责人: Graeme J Wistow
• 金额: $ 115.1万
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7594063
• 关键词: Animal Model; Bioinformatics; Cattle; Collaborations; Complex; Conjunctival Pterygium; Cornea; Custom; DNA; DNA Microarray Chip; DNA Microarray format; Data; Databases; Development; Ear; Essential Genes; Expressed Sequence Tags; Eye; Eye diseases; Florida; Genes; Genetic; Genome; Genomics; Human; Hybrids; Informatics; Libraries; Macaca mulatta; Modeling; Molecular; Mus; Optic Nerve; Oryctolagus cuniculus; Pattern; Protein Analysis; Range; Research; Resources; Sensory; System; Taste Buds; Tissues; Universities; Update; Yeasts; Zebrafish; cDNA Arrays; improved; lens; mouse model; response; tool

Many human eye (and also ear) tissues and those of important animal models have now been analyzed. In addition NEIBank collaborations have added data on other sensory tissues, ear and taste bud. Recent eye additions include human optic nerve, human pterygium, rhesus monkey and cow lens and zebrafish cornea,. This DNA resource has been used to create cDNA microarrays with 13,000 non-redundant human eye expressed sequences and 6500 sequences from mouse eye. These are being used in analysis of human cellular responses and genetically modified mouse models. Data from NEIBank rabbit eye libraries have been used to create the first DNA microarray for rabbit eye research in collaboration with groups from the University of Florida. A powerful addition to the informatics tools for eye research is EyeBrowse, a dedicated genome browser for eye-expressed genes, produced in collaboration with CIT. http://eyebrowse.cit.nih.gov/genome/; a database for known human eye disease genes and a new tool to compare expression patterns of different tissues and species. In addition, NEIBank now incorporates data from the EyeSAGE project (Duke U) with a custom genome browser track for human eye-derived SAGE data. Another addition is a database of human eye disease genes which is currently undergoing update and annotation.

许多人的眼睛（和耳朵）组织和重要的动物模型现在已经被分析。此外，NEIBank的合作增加了其他感觉组织，耳朵和味蕾的数据。最近增加的眼睛包括人类视神经，人类翼状胬肉，恒河猴和牛透镜和斑马鱼角膜。该DNA资源已用于创建具有13，000个非冗余人眼表达序列和6500个来自小鼠眼睛的序列的cDNA微阵列。这些被用于分析人类细胞反应和转基因小鼠模型。来自NEIBank兔眼文库的数据已被用于与佛罗里达大学的小组合作创建第一个用于兔眼研究的DNA微阵列。 眼睛研究信息学工具的一个强大补充是EyeBrowse，这是一个专门用于眼睛表达基因的基因组浏览器，与CIT合作生产。http://eyebrowse.cit.nih.gov/genome/;一个已知人类眼病基因的数据库和一个比较不同组织和物种表达模式的新工具。此外，NEIBank现在将EyeSAGE项目（杜克U）的数据与人类眼睛来源的SAGE数据的自定义基因组浏览器跟踪相结合。另一个补充是人类眼病基因数据库，目前正在进行更新和注释。