NEIBank--EST Analysis/Bioinformatics For Ocular Genomics

NEIBank--眼部基因组学EST分析/生物信息学

• 批准号: 7139191
• 负责人: Graeme J Wistow
• 金额: --
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7139191
• 关键词: animal genetic material tag; animal tissue; bioinformatics; biotechnology; complementary DNA; computer assisted sequence analysis; computer program /software; eye; eye disorder; functional /structural genomics; gene expression; gene expression profiling; genetic library; genetic mapping; genetic registry /resource /referral center; genetically modified animals; human genetic material tag; human tissue; information dissemination; laboratory mouse; microarray technology; molecular biology information system; sequence tagged sites

The eye is a complex system of highly differentiated tissues of various developmental origins. Many genes essential for eye function are tissue-specific and many of those known are associated with genetic eye diseases. The majority of human expressed genes are known only through expressed sequence tags (ESTs). A project called NEIBank was begun to improve the EST coverage and to develop a molecular encyclopedia for the eye (http://neibank.nei.nih.gov). Many human eye (and also ear) tissues and those of important animal models have now been analyzed. Recent additions are human and mouse lacrimal gland, human keratoconus cornea, human pterygium and multiple tissue libraries from dog, rabbit, chicken, guinea pig and zebrafish tissues. The human DNA resource generated by this work has been used to create cDNA microarrays with 13,000 non-redundant human eye expressed sequences and 6500 sequences from mouse eye. A recent addition to the informatics tools for eye research is EyeBrowse, a dedicated genome browser for eye-expressed genes, produced in collaboration with CIT. http://eyebrowse.cit.nih.gov/genome/ ; a database for known human eye disease genes and a new tool to compare expression patterns of different tissues and species.

眼睛是一个复杂的系统，由各种发育起源的高度分化的组织组成。许多对眼睛功能至关重要的基因是组织特异性的，其中许多已知的基因与遗传性眼病有关。大多数人类表达基因只能通过表达序列标签（est）来了解。一个名为NEIBank的项目开始了，以提高EST的覆盖率，并为眼睛开发一个分子百科全书（http://neibank.nei.nih.gov）。许多人的眼睛（和耳朵）组织和重要的动物模型的组织现在已经被分析。最近增加的是人类和小鼠泪腺、人类圆锥角膜、人类翼状胬肉以及来自狗、兔、鸡、豚鼠和斑马鱼组织的多种组织文库。这项工作所产生的人类DNA资源已被用于创建含有13,000个非冗余人眼表达序列和6500个小鼠眼睛表达序列的cDNA微阵列。最近增加的用于眼睛研究的信息学工具是EyeBrowse，这是一种专门用于眼睛表达基因的基因组浏览器，是与CIT. http://eyebrowse.cit.nih.gov/genome/合作生产的；一个已知人类眼病基因的数据库和一个比较不同组织和物种的表达模式的新工具。