NEIBank: Est Analysis And Bioinformatics For Ocular Geno

NEIBank：眼基因的 Est 分析和生物信息学

• 批准号: 7322284
• 负责人: Graeme J Wistow
• 金额: --
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7322284
• 关键词: NEIBank; Est; Analysis; Bioinformatics; Ocular

The eye is a complex system of highly differentiated tissues of various developmental origins. Many genes essential for eye function are tissue-specific and many of those known are associated with genetic eye diseases. The majority of human expressed genes are known only through expressed sequence tags (ESTs). A project called NEIBank was begun to improve the EST coverage and to develop a molecular encyclopedia for the eye (http://neibank.nei.nih.gov). Many human eye (and also ear) tissues and those of important animal models have now been analyzed. Recent additions include human optic nerve, zebrafish cornea and rhesus monkey and cow lens. The human DNA resource generated by this work has been used to create cDNA microarrays with 13,000 non-redundant human eye expressed sequences and 6500 sequences from mouse eye. A powerful addition to the informatics tools for eye research is EyeBrowse, a dedicated genome browser for eye-expressed genes, produced in collaboration with CIT. http://eyebrowse.cit.nih.gov/genome/; a database for known human eye disease genes and a new tool to compare expression patterns of different tissues and species. In addition, NEIBank now incorporates data from the EyeSAGE project (Duke U) with a custom genome browser track for human eye-derived SAGE data. Another addition is a comprehensive database of human eye disease genes.

眼睛是一个复杂的系统，由各种发育起源的高度分化的组织组成。许多对眼功能至关重要的基因是组织特异性的，其中许多已知的基因与遗传性眼病有关。大多数人类表达基因只能通过表达序列标签 (EST) 来了解。一个名为 NEIBank 的项目开始启动，以提高 EST 覆盖范围并开发眼睛分子百科全书 (http://neibank.nei.nih.gov)。现在已经对许多人类眼睛（以及耳朵）组织和重要动物模型的组织进行了分析。最近添加的内容包括人类视神经、斑马鱼角膜以及恒河猴和牛晶状体。这项工作产生的人类 DNA 资源已用于创建 cDNA 微阵列，其中包含 13,000 个非冗余人眼表达序列和 6500 个小鼠眼表达序列。 EyeBrowse 是眼睛研究信息学工具的一个强大补充，它是与 CIT 合作开发的专门用于眼睛表达基因的基因组浏览器。 http://eyebrowse.cit.nih.gov/genome/；已知人类眼部疾病基因的数据库和比较不同组织和物种表达模式的新工具。此外，NEIBank 现在将 EyeSAGE 项目 (Duke U) 的数据与自定义基因组浏览器轨道相结合，以获取人眼衍生的 SAGE 数据。另一个新增内容是人类眼部疾病基因的综合数据库。