NEIBank: Est Analysis And Bioinformatics For Ocular Geno

眼睛是一个复杂的系统，由各种发育起源的高度分化的组织组成。许多对眼睛功能至关重要的基因是组织特异性的，其中许多已知的基因与遗传性眼病有关。大多数人类表达的基因仅通过表达序列标签（EST）已知。开始了一个名为NEIBank的项目，以提高EST的覆盖率，并为眼睛开发一个分子百科全书（neibank.nei.nih.gov）。许多人的眼睛（和耳朵）组织和重要的动物模型现在已经被分析。最近又增加了人类视神经、斑马鱼角膜、恒河猴和牛的透镜。本工作产生的人类DNA资源已用于创建具有13，000个非冗余人眼表达序列和6500个来自小鼠眼睛的序列的cDNA微阵列。眼睛研究信息学工具的一个强大补充是EyeBrowse，这是一个专门用于眼睛表达基因的基因组浏览器，与CIT合作生产。http://eyebrowse.cit.nih.gov/genome/;一个已知人类眼病基因的数据库和一个比较不同组织和物种表达模式的新工具。此外，NEIBank现在将EyeSAGE项目（杜克U）的数据与人类眼睛来源的SAGE数据的自定义基因组浏览器跟踪相结合。另一个补充是人类眼病基因的综合数据库。

The eye is a complex system of highly differentiated tissues of various developmental origins. Many genes essential for eye function are tissue-specific and many of those known are associated with genetic eye diseases. The majority of human expressed genes are known only through expressed sequence tags (ESTs). A project called NEIBank was begun to improve the EST coverage and to develop a molecular encyclopedia for the eye (http://neibank.nei.nih.gov). Many human eye (and also ear) tissues and those of important animal models have now been analyzed. Recent additions include human optic nerve, zebrafish cornea and rhesus monkey and cow lens. The human DNA resource generated by this work has been used to create cDNA microarrays with 13,000 non-redundant human eye expressed sequences and 6500 sequences from mouse eye. A powerful addition to the informatics tools for eye research is EyeBrowse, a dedicated genome browser for eye-expressed genes, produced in collaboration with CIT. http://eyebrowse.cit.nih.gov/genome/; a database for known human eye disease genes and a new tool to compare expression patterns of different tissues and species. In addition, NEIBank now incorporates data from the EyeSAGE project (Duke U) with a custom genome browser track for human eye-derived SAGE data. Another addition is a comprehensive database of human eye disease genes.