Implantable Capsule for Angiogenesis Studies

用于血管生成研究的植入式胶囊

• 批准号: 6228077
• 负责人: Peter M Bungay
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6228077
• 关键词: angiogenesis; biomedical equipment development; fibroblast growth factor; heparin; implant; laboratory mouse; microcapsule; miniature biomedical equipment; slow release drug

The goal of this project is to develop an implantable capsule for studying the promotion and inhibition of angiogenesis in vivo in mice. The approach being pursued employs a tubular capsule sealed at one end and capped with a microporous track-etched membrane at the releasing end. The capsule will be filled with a commercial extracellular matrix gel (e.g., Matrigel) containing angiogenic agents, such as basic fibroblast growth factor and heparin. The capsule configuration was chosen as a means for generating a spatial gradient of the agents within the capsule over a distance of millimeters as a result of diffusional release to the adjacent tissue at the implant site. The intent is to evaluate aspects of the angiogenic response to the released substances, such as the spatial variation in the characteristics and organization of the cells that migrate into the capsules. - angiogenesis, basic fibroblast growth factor, heparin, kinetics, controlled release, gene expression

本项目的目标是开发一种植入性胶囊，用于研究体内促进和抑制小鼠血管生成的作用。正在进行的方法采用管状胶囊，其一端密封，在释放端覆盖有微孔轨道蚀刻膜。该胶囊将填充一种商业细胞外基质凝胶(例如Matrigel)，其中含有血管生成剂，如碱性成纤维细胞生长因子和肝素。由于在植入部位向邻近组织的扩散释放，选择胶囊构型作为在胶囊内产生药剂在毫米距离上的空间梯度的手段。其目的是评估对释放的物质的血管生成反应的各个方面，例如迁移到胶囊中的细胞的特征和组织的空间差异。-血管生成，碱性成纤维细胞生长因子，肝素，动力学，控制释放，基因表达