Implantable Capsule for Angiogenesis Studies

本项目的目标是开发一种可植入的胶囊，用于研究小鼠体内血管生成的促进和抑制作用。所采用的方法采用一端密封的管状胶囊，并在释放端覆盖微孔蚀刻膜。胶囊将填充商业细胞外基质凝胶（例如，Matrigel），其中含有血管生成剂，如碱性成纤维细胞生长因子和肝素。选择胶囊结构作为一种手段，用于在胶囊内产生药物在毫米距离上的空间梯度，因为扩散释放到植入部位的邻近组织。目的是评估对释放物质的血管生成反应的各个方面，例如迁移到胶囊中的细胞的特征和组织的空间变化。-血管生成，碱性成纤维细胞生长因子，肝素，动力学，控释，基因表达

The goal of this project is to develop an implantable capsule for studying the promotion and inhibition of angiogenesis in vivo in mice. The approach being pursued employs a tubular capsule sealed at one end and capped with a microporous track-etched membrane at the releasing end. The capsule will be filled with a commercial extracellular matrix gel (e.g., Matrigel) containing angiogenic agents, such as basic fibroblast growth factor and heparin. The capsule configuration was chosen as a means for generating a spatial gradient of the agents within the capsule over a distance of millimeters as a result of diffusional release to the adjacent tissue at the implant site. The intent is to evaluate aspects of the angiogenic response to the released substances, such as the spatial variation in the characteristics and organization of the cells that migrate into the capsules. - angiogenesis, basic fibroblast growth factor, heparin, kinetics, controlled release, gene expression