Microdialysis Studies

微透析探针技术提供了 用于可扩散组织取样的组织样本 成分或生物活性物质的递送。但 探针灌注液浓度与其组织的关系 对应物是溶质分子量的复函数， 溶质理化性质，组织性质，探针 膜特性、探针几何形状和灌注速率，以及 探针插入组织的创伤。这些依赖关系是 为了提高数据库的定量有用性， 技术.在大脑中的应用正在进行中， 与帕金森病、艾滋病、酗酒和研究的联系 滥用药物。其他应用涉及各种正常组织 和肿瘤。感兴趣的内源性溶质包括多巴胺和 喹啉酸使用的外源性物质的实例是 齐多夫定（AZT）和乙醇。数学建模用于 描述探针内的扩散和对流溶质传输 以及周围组织中的细胞。扩散渗透率和对流渗透率 的探针膜已被确定下 良好表征的体外条件。验证实验， 动物（通常是大鼠）涉及定量放射自显影， 组织学和探针周围组织的化学分析，如 以及探针灌注液浓度的测量。(This是一 校内研究项目Z 01-RR- 10276-10的延续 BEI.）

Microdialysis probe technology provides access to tissue interstitium for either sampling of diffusible tissue constituents or delivery of bioactive substances. However, the relationship of the probe perfusate concentrations to their tissue counterparts is a complex function of solute molecular weight, solute physicochemical properties, tissue properties, probe membrane properties, probe geometry and perfusion rate, and the trauma of probe insertion into the tissue. These dependencies are being studied in order to improve the quantitative usefulness of the technology. Applications in the brain are being pursued in connection with Parkinson's disease, AIDS, alcoholism and studies of drugs of abuse. Other applications involve various normal tissues and tumors. Endogenous solutes of interest include dopamine and quinolinic acid. Examples of exogenous substances employed are Zidovudine (AZT) and ethanol. Mathematical modeling is used to describe diffusive and convective solute transport within the probe and in surrounding tissue. Diffusive and convective permeabilities of probe membranes have been determined under well-characterized conditions in vitro. Validation experiments in animals (usually rats) involve quantitative autoradiography, histology, and chemical assay of tissue surrounding the probe, as well as measurement of probe perfusate concentrations. (This is a continuation of Intramural Research Project Z01-RR- 10276-10 BEI.)