Sub-Diffraction and Sub-Pixel Microscopic Deconvolution

亚衍射和亚像素显微反卷积

• 批准号: 6401366
• 负责人: TIMOTHY J HOLMES
• 金额: $ 10.23万
• 依托单位: LICKENBROCK TECHNOLOGIES, LLC
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6401366
• 关键词: bioimaging /biomedical imaging; computer program /software; computer simulation; computer system design /evaluation; fluorescence microscopy; image processing; light microscopy; optics

DESCRIPTION (provided by applicant): The resolving power of the computer- and video-based light microscope is limited by two main factors. (1) It is limited by the optical resolving power of the microscope, which is -0.25 micro meter (2) It is limited by the pixel size. Resolving power of less than 0.2 micro meter usually requires an electron microscope. Three dimensional imaging to resolving powers of less than 0.2 micro meter requires either an intermediate voltage electron microscope or a high voltage electron microscope (HVEM), both of which are not readily available at most institutions. Live imaging is not possible with any electron microscope. We propose a novel blind deconvolution software algorithm that will commercially provide subpixel resolving power of well under 0.2 micro meter for live fluorescence microscope imaging. The software will be affordable to most biology laboratories and will run on the Windows NT/98 platform. It will require no add-on equipment for the PC or the microscope, which is a critical factor for acceptance by customers and original-equipment-manufacturers through whom the software will be sold. Algorithm research and test bed experimentation of the Phase 1 feasibility will involve verification of subdiffraction 3D structure of the centrosome, a fabricated test target and a computer simulation. PROPOSED COMMERCIAL APPLICATION: Applications include 2D and 3D fluorescence microscopy (FM) and transmitted light brightfield microscopy. Live FM will have a large scientific impact, since presently there is no commercial means for resolving power under 0.2 micrometers. Market inquiries by us indicate that on the order of 400 units per year should be sold by us and an aggregate of at least 3 times this number should be sold by inevitable enduring competitors who will develop competitive products after our product is introduced.

描述（由申请人提供）：计算机的分辨能力， 基于视频的光学显微镜受到两个主要因素的限制。(1)它是有限 通过显微镜的光学分辨能力，即-0.25微米 (2)它受到像素大小的限制。分辨率小于0.2微米 通常需要电子显微镜。三维成像， 小于0.2微米的分辨能力需要一个中间体， 电压电子显微镜或高压电子显微镜（HVEM），两者都 其中大多数机构都不容易获得。实时成像不是 任何电子显微镜都可以。提出了一种新的盲反卷积方法 软件算法，将商业上提供亚像素分辨率的 对于活体荧光显微镜成像而言，在0.2微米以下。的 软件将是负担得起的大多数生物实验室，并将运行在 Windows NT/98平台。它将不需要附加设备的PC或 显微镜，这是客户接受的关键因素， 原始设备制造商，通过他们销售软件。 第一阶段可行性的算法研究和试验台实验将 涉及验证中心体的亚衍射3D结构， 制造的测试目标和计算机模拟。 拟定商业应用： 应用包括2D和3D荧光显微镜（FM）和透射光 明视野显微镜 现场调频将有很大的科学影响，因为目前有 没有任何商业手段可以分辨0.2微米以下的能力。 市场查询 我们表示，我们每年应销售400台， 至少3倍于这个数字应该出售不可避免的持久的竞争对手谁将 在我们的产品推出后开发有竞争力的产品。