NEUROENDOCRINE & GONADAL CONTROL OF MALE REPRODUCTION

神经内分泌

• 批准号: 6076025
• 负责人: William Francis Crowley
• 金额: $ 99.26万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6076025
• 关键词: cooperative study; male reproductive system; neuroendocrine system; reproductive development

DESCRIPTION: (Adapted from the applicant's description) This application for the Harvard-wide Specialized Center for Reproduction proposes to bring the neuroendocrine and gonadal control of reproduction in the male. Two of the component of reproduction while the other two will explore the gonadal level as Leydig cell control of steroidogenesis. In Project I, will employ tandem phenotyping and genotyping approaches in patients with idiopathic hypogonadotropic hypogonadism (IHH). This is a human model in which a series of genetic defects result in failure of the neuroendocrine component of sexual maturation. In Project II, Dr. Ursula Kaiser will explore the GnRH of gonadotropic genes using LH-T2 cells which are of gonadotrope origin. In Inhibiting Substance (MIS) upon steroidogenesis using the CYP17 gene as well MIS receptor. In addition, she will chart the developmental expression of this receptor and correlate it with steroidogenesis. In Project IV, Dr. Joel Habener will continue his explorations of the role of cAMP-activated pathways of alternative splicing of the gene encoding of the transcription factor, CREB. He will also explore the role of pituitary adenyl cyclase activating peptide; PACAP, and its alternatively spliced type 1 receptor in the autocrine control of spermatogenesis.

描述：（改编自申请人的描述）哈佛生殖专业中心的这项申请提议将神经内分泌和性腺控制男性的生殖。其中两个是生殖的组成部分，另外两个将探索性腺水平作为间质细胞控制类固醇生成的作用。在项目 I 中，将对特发性低促性腺素性性腺功能减退症 (IHH) 患者采用串联表型和基因分型方法。这是一个人类模型，其中一系列遗传缺陷导致性成熟的神经内分泌部分失败。在项目 II 中，Ursula Kaiser 博士将利用促性腺激素来源的 LH-T2 细胞探索促性腺激素基因的 GnRH。使用 CYP17 基因以及 MIS 受体抑制类固醇生成物质 (MIS)。此外，她还将绘制该受体的发育表达图，并将其与类固醇生成相关联。在项目 IV 中，Joel Habener 博士将继续探索 cAMP 激活途径对转录因子 CREB ​​编码基因选择性剪接的作用。他还将探索垂体腺苷酸环化酶激活肽的作用； PACAP 及其选择性剪接 1 型受体在精子发生自分泌控制中的作用。