RENAL BASIS FOR HYPOCITRATURIA

低柠檬酸尿症的肾脏基础

• 批准号: 6335259
• 负责人: ROBERT J ALPERN
• 金额: $ 0.7万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2001-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6335259
• 关键词: Mammalia; biomedical resource; carbohydrates; cardiovascular system; enzymes; nuclear magnetic resonance spectroscopy; technology /technique

Dr. Taegtmeyer's group continues to examine mobilization of glycogen and glycogen turnover during normoxia and myocardial ischemia. The goal is to use 13C NMR isotopomer analysis to monitor the contribution of glycogen to acetyl-CoA and energy generation. The basic protocol uses depletion followed by glycogen repletion with 13C enriched glycosyl units. Tissue extracts from experiments performed in Houston were analyzed at the Resource and demonstrated that lactate, glutamate and glucose could be easily detected. However, with the very brief (3 min) period for citric acid cycle turnover plus the low enrichment inherent in the use of [1-13C] glucose, 13C-13C spin-spin coupling could not be detected. These experiments were redesigned with [1,6-13C] glucose and a longer period for cycle turnover which should yield spectra suitable for analysis. (Collaborative 7) REPORT PERIOD: (09/01/97-08/31/98)

Taegtmeyer博士的研究小组继续研究 常氧和心肌缺血期间的糖原和糖原代谢 缺血 目标是使用13 C NMR同位素分析来监测 糖原对乙酰辅酶A和能量产生的贡献。 的 基本方案使用消耗，然后用13 C补充糖原 富集的糖基单元。 进行的实验的组织提取物 在休斯顿进行了分析，并证明， 乳酸盐、谷氨酸盐和葡萄糖可以容易地检测。 然而，在这方面， 柠檬酸循环周转时间非常短（3分钟）， 使用[1- 13 C]葡萄糖、13 C-13 C 不能检测到自旋-自旋耦合。 这些实验 使用[1，6 - 13 C]葡萄糖重新设计，周期更长 转换，这应该产生适合于分析的光谱。 （协作7）报告期：（1997年9月1日至1998年8月31日）