ULTRAFAST TEMPERATURE JUMP METHODS

高能激光脉冲(300？J-2 MJ)被用来激发 溶液中的吸附染料。这些染料起到了热传感器的作用， 将光能转化为热能。这 热能以约100ps的速度在整个主体溶剂中扩散， 将溶剂的温度提高到8-100摄氏度左右 利用和探索进一步的光学参数方法 产生可被直接吸收到泛音中的脉冲 溶剂水的谱带(约1.5-1.9微米)。温度的跳跃是 用于诱导与展开相关的构象变化 在蛋白质中。超快红外和可见光探测器已经被 开发的目的是监测这些变化的动力学。进一步 已经对系统进行了修改，以允许观察 PS到ms时间尺度上的光谱变化。其他候选蛋白质 正在考虑中，特别是那些经历了寒冷的人 “自然”，以便我们可以观察到与 折叠过程。

Energetic laser pulses (300 ?J- 2 mJ) are being utilized to excite absorptive dyes in solution. These dyes act as heat transducers, taking the optical energy and converting it to thermal energy. This thermal energy diffuses throughout the bulk solvent in ca. 100 ps, raising the temperature of the solvent on the order of 8-100 C. We are utilizing and exploring further optical parametric methods of generating the pulses that can be directly absorbed into the overtone bands of the solvent water (ca. 1.5-1.9 ?m). The temperature jump is being used to induce conformational changes associated with unfolding in proteins. Ultrafast infrared and visible probes have been developed to monitor the kinetics of these changes. Further modifications have been made to the system to allow observation of spectral changes on the ps to ms timescale. Other protein candidates are being considered, in particular those which undergo cold "naturation" such that we may observe changes associated with the folding processes.