ULTRAFAST TEMPERATURE JUMP METHODS

超快温度跳跃方法

• 批准号: 6328061
• 负责人: C M PHILLIPS
• 金额: $ 1.64万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6328061
• 关键词: biological products; biomedical resource; lasers; proteins

Energetic laser pulses (300 ?J- 2 mJ) are being utilized to excite absorptive dyes in solution. These dyes act as heat transducers, taking the optical energy and converting it to thermal energy. This thermal energy diffuses throughout the bulk solvent in ca. 100 ps, raising the temperature of the solvent on the order of 8-100 C. We are utilizing and exploring further optical parametric methods of generating the pulses that can be directly absorbed into the overtone bands of the solvent water (ca. 1.5-1.9 ?m). The temperature jump is being used to induce conformational changes associated with unfolding in proteins. Ultrafast infrared and visible probes have been developed to monitor the kinetics of these changes. Further modifications have been made to the system to allow observation of spectral changes on the ps to ms timescale. Other protein candidates are being considered, in particular those which undergo cold "naturation" such that we may observe changes associated with the folding processes.

高能激光脉冲（300？J- 2 mJ）被用来激发 溶液中的吸收性染料。 这些染料充当热转换器， 吸收光能并将其转化为热能。 这 热能在大约100 ℃下扩散到整个本体溶剂中。 100 ps， 将溶剂的温度升高8-100 ℃。我们 利用和探索进一步的光学参数方法， 产生可直接吸收到泛音中的脉冲 溶剂水的谱带（ca. 1.5-1.9？m）。 温度的跳跃是 用于诱导与解折叠相关的构象变化 在蛋白质中。 超快红外和可见光探测器已经被 来监测这些变化的动力学。 进一步 对系统进行了修改，以允许观察 在ps到ms时间尺度上的光谱变化。 其他候选蛋白质 特别是那些经历过寒冷天气的人， “自然化”，这样我们就可以观察到与 折叠过程