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SITE SPECIFIC GENE INSERTION BY TRANSPOSITION

SITE SPECIFIC GENE INSERTION BY TRANSPOSITION
通过转座进行位点特异性基因插入
批准号：
6342058
负责人：
ERIC WICKSTROM
金额：
$24.07万
依托单位：
THOMAS JEFFERSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
项目状态：
已结题
起止时间：
1999-01-01 至 2003-12-31
来源：
https://reporter.nih.gov/project-details/6342058
关键词：
bacterial proteins cell free system chromatography chromosomes electroporation eukaryote fungal genetics gene expression gene therapy genes genetically modified animals genome laboratory mouse microinjections nucleic acid sequence plasmids protein binding reporter genes site directed mutagenesis tissue /cell culture transfection /expression vector transposon /insertion element yeasts

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英文摘要
DESCRIPTION (Applicant's Abstract): The central hypothesis of this proposal is that site and sequence specific insertion of genes into eukaryotic chromosomes can be carried out with Tn7 transpositional proteins. The overall objective is to establish a biological basis for gene therapy based on the use of transposon Tn7 proteins. Tn7 was selected for its ability to integrate into a DNA sequence with specificity and efficiency. Preliminary data support a hypothesis that Tn7 based insertion could provide a safe and efficient method to incorporate therapeutic genes into chromosomes. The central hypothesis will be tested in three Specific Aims: 1.Will Tn7 proteins transposed into eukaryotic targets in vitro? 2. Will Tn7 proteins transpose into eukaryotic targets in vivo? 3. Will Tn7 transposition disrupt normal glutamine-fructose-6-phosphate transaminase (GFPT) expression?
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