SENSITIVE AND SELECTIVE DETECTION OF PEPTIDES

中风、窒息、勒死、头部受伤和相关事件造成的大脑损伤以一种显著的方式发生。大部分损害是延迟的，在事件发生后数小时或数天发生，为药物治疗方法留下了充足的时间，以尽量减少损害。已知细胞延迟死亡是化学诱导的，神经递质对神经细胞的过度兴奋导致细胞死亡。我们还知道，多肽常常控制神经对神经递质的敏感性。肽是否通过使细胞对神经递质高度敏感而在细胞死亡中起作用？肽类药物可以用来降低神经的敏感性吗？如果没有技术的进步，这些问题很难回答。提出了两项主要进展。其一，目前效率低下的大脑多肽取样将得到改进。另一方面，分离/检测系统将开发，将允许快速，敏感和选择性检测肽及其代谢物。这些技术将应用于大鼠缺血模型。

Damage to the brain resulting from stroke, asphyxiation, strangulation, head injury and related events occur in a remarkable way. Much of the damage is delayed, occurring hours or days following the incident, leaving plenty of time for pharmacotherapeutic approaches to minimize damage. It is known that the delayed cell death is chemically induced, and that overexcitation of nerve cells by neurotransmitters leads to cell death. It is also known that peptides often control a nerve s sensitivity to neurotransmitters. Do peptides play a role in cell death by causing them to be highly sensitive to neurotransmitters? Could peptide-like drugs be used to lower a nerve s sensitivity? These questions are difficult to answer without advances in technology. Two major advances are proposed. In one, the sampling of peptides from the brain, now inefficient, will be improved. In another, separation/detection systems will be developed that will allow for rapid, sensitive and selective detection of peptides and their metabolites. These techniques will be applied in rat models of ischemia.