PERMSELECTIVITY OF MYOCARDIAL CAPILLARIES (EFFECTS OF ELECTROKINETICS)

心肌毛细血管的通透性（电动效应）

• 批准号: 6308532
• 负责人: JAMES B BASSINGTHWAIGHTE
• 金额: $ 2.35万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6308532
• 关键词: Mammalia; bioengineering /biomedical engineering; biomedical resource; human tissue; technology /technique

An idealized continuum model of electrokinetic transport is developed to analyze the contributions of solute and barrier charge to microvascular permeability. The glycocalyx fiber-matrix-filled interendothelial cleft of the transcapillary exchange barrier is modelled with parallel plates representing endothelial cell surfaces and a bridging regular array of identical cylinders representing glycocalyx fibers. To calculate the electrostatic potential within the confined fiber-matrix, the linear Poisson-Boltzmann equation is solved for a single bounded post and a regular array of bounded posts using several analytical methods (separation of variables, Green's functions and linear superposition and unit cell approximations) and numerical methods (direct formulation of a boundary element method and boundary collocation method). The potential decays monotonically with distance from the surfaces vanishing between four and five Debye lengths. For values typical of plasma ultrafiltrate in a glycocalyx filled interendothelial cleft, namely a Debye length of 8 A , plate separation of 120 A, post radius of 6 A and surface potential of 60 mV, the potential remains nonzero for porosity values less than 97%. Thus, throughout the confined matrix region of this idealized model, there is enhanced anionic and diminished cationic convective transport compared to neutral molecules. The effect is more pronounced for larger molecules whose double layers extend into double layer regions close to the post or plates where the potential magnitude is greatest. This result agrees with published experimental observations of transport of charged molecules.

电动输运的理想化连续介质模型是 用来分析溶质和势垒电荷对 微血管通透性。糖萼纤维基质填充物 跨毛细血管交换屏障的内皮间裂隙 用代表内皮细胞表面的平行板建模 以及相同圆柱体的桥接规则阵列，该圆柱体表示 糖萼纤维。要计算内部的静电势 对于受限纤维基质，线性泊松-玻尔兹曼方程是 单个有界柱和有界柱的规则阵列的解 使用几种分析方法(分离变量、格林的 函数、线性叠加和单位元胞近似)和 数值方法(边界元方法的直接公式和 边界配置法)。电势随时间单调衰减 从四号到五号德拜之间消失的表面的距离 长度。了解糖基化反应中血浆超滤液的典型数值 填充性内皮间裂隙，即德拜长度为8A的钢板 间隔120A，柱子半径6A，表面电位60 MV，当孔隙度小于97%时，电势保持非零值。 因此，在该理想化模型的受限矩阵区域中， 阴离子对流输送增强，阳离子对流输送减弱 与中性分子相比。这种影响在以下方面更为明显 双层延伸到双层区域的较大分子 靠近潜在震级最大的柱子或板块。 这一结果与发表的实验观察结果一致。 带电分子的运输。