AN AUTOMATED MICRO-IMAGING FACILITY

自动化显微成像设备

• 批准号: 6291350
• 负责人: BASIL V WORGUL
• 金额: $ 14.35万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2002-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291350
• 关键词: biomedical equipment purchase; confocal scanning microscopy; protein localization

The use of powerful and rapidly developing high resolution imaging techniques (e.g. the development of the green (and other) fluorescent protein markers or fusions, optical sectioning, sample sparing multi- photon excitation, fluorescence resonance energy transfer (FRET) analyses, and newer fluorophores) has allowed information on the localization and interactions of proteins much more accessible to a large segment of the research community. Such techniques are quickly becoming standard methodologies for most basic scientists, not just cell biologists. The goal of this proposal is to provide ready access for members of (primarily) the Department of Biochemistry at Emory University to state-of-the-art multi-photon, UV, and laser scanning confocal microscopy. Access to the proposed instrument will allow technique development as well as having an immediate positive impact on the ability to obtain certain types and quality of other types of data readily achieved in at least eight laboratories (5 major and 3 minor users) currently funded by the NIH to the extent of more than $2.2M direct annual costs. Three principal goals will be achieved with the purchase of this instrument; it will (1) allow ready access of a group of well-funded investigators to high resolution confocal microscopy for detailed protein localization, FRET, and quantitative approaches to biological problems, (2) provide for multi-photon excitation imaging of samples to minimize photodamage, photobleaching, and allow deeper penetration of tissue sections, and (3) extend into the UV range for illumination capabilities to extend the types of chromophores, FRET pairs, and multiple channel recordings available to investigators. Goals #2 and #3 are not possible with any instrumentation currently available at Emory University.

使用强大且快速发展的高分辨率成像技术（例如，绿色（和其他）荧光蛋白标记或融合的发展，光学切片，样品较高的多光子激发，荧光共振能量转移（FRET）分析以及较新的元素允许对蛋白质的本地化和相互作用的信息进行更多的研究，以供蛋白质的本地化和相互作用。对于大多数基本科学家而言，这种技术正在迅速成为标准方法，而不仅仅是细胞生物学家。该提案的目的是为埃默里大学生物化学系的成员提供现成的访问权限，以使最先进的多光量，紫外线和激光扫描共共聚焦显微镜。访问提议的工具将允许技术开发，并立即对获得NIH目前由NIH资助的至少八个实验室（5名主要和3个次要用户）获得的其他类型的数据的某些类型和质量产生积极的影响，其范围超过220万美元。购买该工具将实现三个主要目标； （1）将允许一组资助良好的研究者准备好高分辨率共焦显微镜，以详细的蛋白质定位，FRET和定量方法来解决生物问题，（2）提供样品的多光子激发成像，以最大程度地减少光损伤，光漂白，并延伸到更深入的范围，以扩展到unum toffers intermantion for intermantion intermantion for intermantion for in toffer intermantion for in toffer inter inter inter（3）uv infrum informing inter（3）调查人员可供研究者使用发色团，fret对和多个通道记录。埃默里大学目前可用的任何仪器都无法实现目标＃2和＃3。