EFFECTS OF ALGINATE COMPOSITION ON ENCAPSULATED CELLS

藻酸盐组合物对封装细胞的影响

• 批准号: 6287670
• 负责人: IOANNIS CONSTANTINIDIS
• 金额: $ 33.44万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2002-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6287670
• 关键词: NOD mouse; alginates; animal tissue; artificial endocrine pancreas; bioengineering /biomedical engineering; biomaterial evaluation; cell cell interaction; cell growth regulation; cell population study; diabetes mellitus therapy; extracellular matrix; insulin; insulin dependent diabetes mellitus; microcapsule; nuclear magnetic resonance spectroscopy; pancreatic islet function; swine; technology /technique development; tissue engineering

DESCRIPTION: (verbatim) Use of alginates to encapsulate cells in order to provide mechanical support and immunoprotection is common practice in the development of a bioartificial pancreas. Alginate is a family of linear binary heteropolymers composed of a-L-guluronic acid and b-D-mannuronic acid residues in various proportion, sequence, and molecular weight. Given their variety in composition, it is our hypothesis that the behavior of encapsulated cells will depend on the composition of the alginate matrix. It is our objective to study the interaction between alginate matrix and encapsulated cells in model tissue-engineered pancreatic constructs. These model constructs are composed of neonatal porcine islets or mouse insulinoma bTC3 cells encapsulated in one of four different types of alginate. The specific aims of the proposed research are to determine the property of alginate gel responsible for affecting the behavior of encapsulated cells, and conversely to investigate the affect of encapsulated cells on the alginate matrix, in vitro and in vivo. To complete the first specific aim, the metabolic and secretory profiles of the encapsulated cells will be monitored, and the apoptotic, proliferative, viable and necrotic cell fractions determined. To complete the second specific aim, 1H NMR techniques will be employed to monitor changes in the alginate matrix. Overall, the proposed experiments are a thorough investigation of the interaction between alginate, as an extracellular matrix, and encapsulated cells. The criteria to judge this interaction are: insulin secretion; bead integrity; cell growth; and in vivo stability. It is anticipated that we will identify alginate compositions that are beneficial to a tissue-engineered pancreas as well as compositions that are inappropriate. Furthermore, the proposed NMR experiments will enhance our understanding of the capabilities of NMR to monitor a tissue-engineered construct in vitro and in vivo.

描述：（逐字）使用海藻酸盐包封细胞，以便