EFFECTS OF ALGINATE COMPOSITION ON ENCAPSULATED CELLS

藻酸盐组合物对封装细胞的影响

• 批准号: 6699610
• 负责人: IOANNIS CONSTANTINIDIS
• 金额: $ 29.7万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6699610
• 关键词: NOD mouse; alginates; animal tissue; artificial endocrine pancreas; bioengineering /biomedical engineering; biomaterial evaluation; cell cell interaction; cell growth regulation; cell population study; diabetes mellitus therapy; extracellular matrix; insulin; insulin dependent diabetes mellitus; microcapsule; nuclear magnetic resonance spectroscopy; pancreatic islet function; swine; technology /technique development; tissue engineering

DESCRIPTION: (verbatim) Use of alginates to encapsulate cells in order to provide mechanical support and immunoprotection is common practice in the development of a bioartificial pancreas. Alginate is a family of linear binary heteropolymers composed of a-L-guluronic acid and b-D-mannuronic acid residues in various proportion, sequence, and molecular weight. Given their variety in composition, it is our hypothesis that the behavior of encapsulated cells will depend on the composition of the alginate matrix. It is our objective to study the interaction between alginate matrix and encapsulated cells in model tissue-engineered pancreatic constructs. These model constructs are composed of neonatal porcine islets or mouse insulinoma bTC3 cells encapsulated in one of four different types of alginate. The specific aims of the proposed research are to determine the property of alginate gel responsible for affecting the behavior of encapsulated cells, and conversely to investigate the affect of encapsulated cells on the alginate matrix, in vitro and in vivo. To complete the first specific aim, the metabolic and secretory profiles of the encapsulated cells will be monitored, and the apoptotic, proliferative, viable and necrotic cell fractions determined. To complete the second specific aim, 1H NMR techniques will be employed to monitor changes in the alginate matrix. Overall, the proposed experiments are a thorough investigation of the interaction between alginate, as an extracellular matrix, and encapsulated cells. The criteria to judge this interaction are: insulin secretion; bead integrity; cell growth; and in vivo stability. It is anticipated that we will identify alginate compositions that are beneficial to a tissue-engineered pancreas as well as compositions that are inappropriate. Furthermore, the proposed NMR experiments will enhance our understanding of the capabilities of NMR to monitor a tissue-engineered construct in vitro and in vivo.

描述：（逐字）使用藻酸盐包封细胞， 提供机械支持和免疫保护是常见的做法， 生物人工胰腺的发展。藻酸盐是一个线性双星家族 由a-L-古罗糖醛酸和b-D-甘露糖醛酸残基组成的杂聚物 不同比例、顺序和分子量。考虑到它们在 组成，这是我们的假设，封装细胞的行为将 取决于藻酸盐基质的组成。我们的目标是研究 海藻酸钠基质与微囊化细胞的相互作用 组织工程胰腺结构。这些模型结构由以下组成 新生猪胰岛或小鼠胰岛素瘤bTC 3细胞包封在 四种不同的海藻酸盐拟议研究的具体目标 是为了确定海藻酸盐凝胶的性质，负责影响 行为的封装细胞，并反过来调查的影响， 在体外和体内，将细胞包封在藻酸盐基质上。完成 第一个具体的目标，代谢和分泌概况的 将监测包封的细胞，并且将检测凋亡的、增殖的、存活的 并测定坏死细胞分数。为了完成第二个具体目标，1H 将采用NMR技术监测藻酸盐基质的变化。 总的来说，所提出的实验是对 藻酸盐作为细胞外基质与包封物之间相互作用 细胞判断这种相互作用的标准是：胰岛素分泌;珠 完整性;细胞生长;和体内稳定性。预计我们将 鉴定有益于组织工程化 胰腺以及不合适的成分。而且 提出的核磁共振实验将提高我们的能力的理解， NMR监测体外和体内的组织工程构建体。