EFFECTS OF ALGINATE COMPOSITION ON ENCAPSULATED CELLS

藻酸盐组合物对封装细胞的影响

• 批准号: 6682842
• 负责人: IOANNIS CONSTANTINIDIS
• 金额: $ 25.59万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6682842
• 关键词: NOD mouse; alginates; animal tissue; artificial endocrine pancreas; bioengineering /biomedical engineering; biomaterial evaluation; cell cell interaction; cell growth regulation; cell population study; diabetes mellitus therapy; extracellular matrix; insulin; insulin dependent diabetes mellitus; microcapsule; nuclear magnetic resonance spectroscopy; pancreatic islet function; swine; technology /technique development; tissue engineering

DESCRIPTION: (verbatim) Use of alginates to encapsulate cells in order to provide mechanical support and immunoprotection is common practice in the development of a bioartificial pancreas. Alginate is a family of linear binary heteropolymers composed of a-L-guluronic acid and b-D-mannuronic acid residues in various proportion, sequence, and molecular weight. Given their variety in composition, it is our hypothesis that the behavior of encapsulated cells will depend on the composition of the alginate matrix. It is our objective to study the interaction between alginate matrix and encapsulated cells in model tissue-engineered pancreatic constructs. These model constructs are composed of neonatal porcine islets or mouse insulinoma bTC3 cells encapsulated in one of four different types of alginate. The specific aims of the proposed research are to determine the property of alginate gel responsible for affecting the behavior of encapsulated cells, and conversely to investigate the affect of encapsulated cells on the alginate matrix, in vitro and in vivo. To complete the first specific aim, the metabolic and secretory profiles of the encapsulated cells will be monitored, and the apoptotic, proliferative, viable and necrotic cell fractions determined. To complete the second specific aim, 1H NMR techniques will be employed to monitor changes in the alginate matrix. Overall, the proposed experiments are a thorough investigation of the interaction between alginate, as an extracellular matrix, and encapsulated cells. The criteria to judge this interaction are: insulin secretion; bead integrity; cell growth; and in vivo stability. It is anticipated that we will identify alginate compositions that are beneficial to a tissue-engineered pancreas as well as compositions that are inappropriate. Furthermore, the proposed NMR experiments will enhance our understanding of the capabilities of NMR to monitor a tissue-engineered construct in vitro and in vivo.

描述：(逐字逐句地)使用藻酸盐包裹细胞，以便 提供机械支持和免疫保护是 生物人工胰腺的开发。藻酸盐是一族线性二元组 A-L-古糖醛酸和b-D-甘露糖酸残基组成的杂化聚合物 以不同的比例、顺序和相对分子质量。考虑到它们的多样性 组成，我们假设封装的细胞的行为将 取决于藻酸盐基质的组成。我们的目标是研究 藻酸盐基质与微囊化细胞在模型中的相互作用 组织工程化的胰腺结构。这些模型构造由以下部分组成 新生猪胰岛或小鼠胰岛素瘤bTC3细胞被包裹在 四种不同类型的海藻酸盐。拟议研究的具体目标 是为了确定海藻酸凝胶的性质，这是影响 封装细胞的行为，并反过来研究 微囊化细胞在藻酸盐基质上，在体外和体内。要完成 第一个特定的目标，新陈代谢和分泌情况 被包裹的细胞将被监测，并且凋亡、增殖、存活 并确定了坏死细胞的比例。要完成第二个具体目标，1H 核磁共振技术将被用来监测藻酸盐基质的变化。 总体而言，拟议的实验是对 藻酸盐作为细胞外基质与微囊化的相互作用 细胞。判断这种相互作用的标准是：胰岛素分泌；珠子 完整性；细胞生长；体内稳定性。预计我们将 鉴定对组织工程有益的藻酸盐组合物 胰腺以及不适当的成分。此外， 拟议的核磁共振实验将增强我们对核磁共振能力的理解 核磁共振用于在体外和体内监测组织工程构建。