EFFECTS OF ALGINATE COMPOSITION ON ENCAPSULATED CELLS

藻酸盐组合物对封装细胞的影响

• 批准号: 6682842
• 负责人: IOANNIS CONSTANTINIDIS
• 金额: $ 25.59万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6682842
• 关键词: NOD mouse; alginates; animal tissue; artificial endocrine pancreas; bioengineering /biomedical engineering; biomaterial evaluation; cell cell interaction; cell growth regulation; cell population study; diabetes mellitus therapy; extracellular matrix; insulin; insulin dependent diabetes mellitus; microcapsule; nuclear magnetic resonance spectroscopy; pancreatic islet function; swine; technology /technique development; tissue engineering

DESCRIPTION: (verbatim) Use of alginates to encapsulate cells in order to provide mechanical support and immunoprotection is common practice in the development of a bioartificial pancreas. Alginate is a family of linear binary heteropolymers composed of a-L-guluronic acid and b-D-mannuronic acid residues in various proportion, sequence, and molecular weight. Given their variety in composition, it is our hypothesis that the behavior of encapsulated cells will depend on the composition of the alginate matrix. It is our objective to study the interaction between alginate matrix and encapsulated cells in model tissue-engineered pancreatic constructs. These model constructs are composed of neonatal porcine islets or mouse insulinoma bTC3 cells encapsulated in one of four different types of alginate. The specific aims of the proposed research are to determine the property of alginate gel responsible for affecting the behavior of encapsulated cells, and conversely to investigate the affect of encapsulated cells on the alginate matrix, in vitro and in vivo. To complete the first specific aim, the metabolic and secretory profiles of the encapsulated cells will be monitored, and the apoptotic, proliferative, viable and necrotic cell fractions determined. To complete the second specific aim, 1H NMR techniques will be employed to monitor changes in the alginate matrix. Overall, the proposed experiments are a thorough investigation of the interaction between alginate, as an extracellular matrix, and encapsulated cells. The criteria to judge this interaction are: insulin secretion; bead integrity; cell growth; and in vivo stability. It is anticipated that we will identify alginate compositions that are beneficial to a tissue-engineered pancreas as well as compositions that are inappropriate. Furthermore, the proposed NMR experiments will enhance our understanding of the capabilities of NMR to monitor a tissue-engineered construct in vitro and in vivo.

描述：（逐字）使用藻酸盐封装细胞以便为了 提供机械支持和免疫保护是常见的实践 生物人工胰腺的开发。藻酸盐是线性二进制的家族 由a-l-guluronic酸和b-d-甘露烯酸残基组成的杂聚物 以各种比例，序列和分子量。鉴于他们的多样性 组成，我们的假设是，封装细胞的行为将 取决于藻酸盐基质的组成。我们的目标是学习 藻酸盐基质与模型中封装的细胞之间的相互作用 组织设计的胰腺结构。这些模型结构由 新生儿猪胰岛或小鼠胰岛素瘤BTC3细胞封装在其中一个 四种不同类型的藻酸盐。拟议研究的具体目的 确定藻酸盐凝胶的特性负责影响 封装细胞的行为，相反是为了研究 在体外和体内封装在藻酸盐基质上的细胞。完成 第一个特定目标，代谢和分泌概况 将监控封装的细胞，并凋亡，增殖，生存 和坏死细胞分数确定。要完成第二个特定目标，1H NMR技术将用于监视藻酸盐矩阵中的变化。 总体而言，提出的实验是对 藻酸盐之间的相互作用，作为细胞外基质并封装 细胞。判断这种互动的标准是：胰岛素分泌；珠子 正直;细胞生长；和体内稳定性。预计我们会 识别有益于组织工程的藻酸盐组成 胰腺以及不合适的成分。此外， 提出的NMR实验将增强我们对 NMR在体外和体内监测组织工程的构建体。