MR STUDIES OF ALGINATE ENCAPSULATED CELLS

海藻酸盐封装细胞的 MR 研究

• 批准号: 7369578
• 负责人: IOANNIS CONSTANTINIDIS
• 金额: $ 0.58万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369578
• 关键词: MR; STUDIES; ALGINATE; ENCAPSULATED; CELLS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In an effort to cure diabetes, one promising development is the implantation of gel encapsulated cells that can produce insulin. These encapsulated cells can then be implanted in patients. We are developing a variety of encapsulation methods and are using MR both on encapsulated cell samples and in vivo in animal models in order to evaluate different systems and methods. In particular, we are presently evaluating a variety of alginate combinations to generate alginate beads with different properties and characteristics and evaluating their ability to support different cell types. A publication describing this work on beads of differing alginate compositions has now been published. Additionally we continue to examine the MR characteristics of beads implanted with pancreatic islets. Most recently we obtained the first MR images of human islets. For these studies we have integrated the microcoils with a perfusion system to keep the tissues viable in the instrument. The images show a clear heterogeneity within the beads. We are in the process of reproducing these results and determining if they provide information relevant with respect to tissue viability. These data were used in the submission of an NIH grant on microimaging studies of the beads (PI = Dr Constantinidis).

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。在治愈糖尿病的努力中，一个有希望的发展是植入可以产生胰岛素的凝胶包裹细胞。然后可以将这些包封的细胞植入患者体内。我们正在开发各种包封方法，并在包封的细胞样品和动物模型体内使用MR，以评估不同的系统和方法。特别是，我们目前正在评估各种藻酸盐组合，以产生具有不同性质和特征的藻酸盐珠，并评估其支持不同细胞类型的能力。现在已经出版了描述关于不同藻酸盐组合物的珠粒的这项工作的出版物。 此外，我们继续检查植入胰岛的珠的MR特征。最近，我们获得了人类胰岛的第一个MR图像。在这些研究中，我们将微弹簧圈与灌注系统集成在一起，以保持组织在仪器中存活。图像显示珠粒内存在明显的异质性。我们正在重现这些结果，并确定它们是否提供了与组织活力相关的信息。这些数据被用于提交NIH资助的微珠显微成像研究（PI = Constantinovi博士）。