Novel mammalian midbody proteins by MALDI-MS

MALDI-MS 发现的新型哺乳动物中间体蛋白

• 批准号: 6404503
• 负责人: Ahna Renee Skop
• 金额: $ 3.48万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6404503
• 关键词: Xenopus; Xenopus oocyte; cell cycle; cell cycle proteins; cell line; matrix assisted laser desorption ionization; mitotic spindle apparatus; postdoctoral investigator; protein localization

The goal of this project is to identify and characterize proteins that comprise the mammalian midbody by matrix assisted laser desorption/ionization (MALDI-MS) to identify novel players during mitotic spindle assembly and cytokinesis. I plan to screen Xenopus spindle extracts for proteins that may affect spindle assembly. I will utilize the C. elegans system to look for proteins that affect cytokinesis utilizing live imaging and RNA interference techniques. In addition, C. elegans and human cell lines will provide excellent systems to study the localization of these proteins during mitosis. Ultimately, I hope to address questions of cellular organization and cytokinesis as an independent investigator at an academic or research institute. Since many cell division processes are perturbed in cancerous cells and birth defects, the understanding of the mechanisms that underlie cell division processes ought to lead to an understanding of the basis for cancer pathology. In the post-genomic era, the discovery of proteins that comprise cellular structures, proteomics, will aid in drug development since all drugs are directed against proteins.

该项目的目标是通过基质辅助激光解吸/电离（MALDI-MS）识别和表征组成哺乳动物中间体的蛋白质，以识别有丝分裂纺锤体组装和胞质分裂过程中的新型参与者。我计划筛选非洲爪蟾纺锤体提取物中可能影响纺锤体组装的蛋白质。我将使用C。elegans系统寻找蛋白质，影响胞质分裂利用活成像和RNA干扰技术。此外，C.线虫和人类细胞系将提供极好的系统来研究这些蛋白质在有丝分裂期间的定位。最后，我希望在学术或研究机构作为一个独立的调查员解决细胞组织和胞质分裂的问题。由于许多细胞分裂过程在癌细胞和出生缺陷中受到干扰，对细胞分裂过程机制的理解应该导致对癌症病理学基础的理解。在后基因组时代，蛋白质的发现，包括细胞结构，蛋白质组学，将有助于药物开发，因为所有的药物都是针对蛋白质。