Novel mammalian midbody proteins by MALDI-MS

该项目的目标是通过基质辅助激光解吸/电离(MALDI-MS)识别和表征组成哺乳动物中体的蛋白质，以识别有丝分裂纺锤体组装和胞质分裂过程中的新成员。我计划筛选非洲爪哇纺锤体提取物中可能影响纺锤体组装的蛋白质。我将利用线虫系统，利用实时成像和RNA干扰技术寻找影响细胞质分裂的蛋白质。此外，线虫和人类细胞系将提供极好的系统来研究这些蛋白质在有丝分裂过程中的定位。最终，我希望作为一名学术或研究机构的独立研究员来解决细胞组织和细胞质分裂的问题。由于许多细胞分裂过程在癌细胞和先天缺陷中受到干扰，因此对细胞分裂过程背后的机制的理解应该有助于理解癌症病理学的基础。在后基因组时代，组成细胞结构的蛋白质的发现，蛋白质组学，将有助于药物开发，因为所有药物都是针对蛋白质的。

The goal of this project is to identify and characterize proteins that comprise the mammalian midbody by matrix assisted laser desorption/ionization (MALDI-MS) to identify novel players during mitotic spindle assembly and cytokinesis. I plan to screen Xenopus spindle extracts for proteins that may affect spindle assembly. I will utilize the C. elegans system to look for proteins that affect cytokinesis utilizing live imaging and RNA interference techniques. In addition, C. elegans and human cell lines will provide excellent systems to study the localization of these proteins during mitosis. Ultimately, I hope to address questions of cellular organization and cytokinesis as an independent investigator at an academic or research institute. Since many cell division processes are perturbed in cancerous cells and birth defects, the understanding of the mechanisms that underlie cell division processes ought to lead to an understanding of the basis for cancer pathology. In the post-genomic era, the discovery of proteins that comprise cellular structures, proteomics, will aid in drug development since all drugs are directed against proteins.