Regulation of Juvenile Hormone levels in mosquitos
蚊子保幼激素水平的调节
基本信息
- 批准号:6382658
- 负责人:
- 金额:$ 29.79万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-07-01 至 2004-06-30
- 项目状态:已结题
- 来源:
- 关键词:Aedes biological signal transduction ganglions gas chromatography mass spectrometry gene expression hormone biosynthesis hormone regulation /control mechanism juvenile hormones microarray technology neuroendocrine system neuropeptides neuroregulation northern blottings nutrition nutrition related tag radiochemistry radioimmunoassay trypsin
项目摘要
DESCRIPTION (provided by the applicant): Mosquito-transmitted parasitic
diseases are among the major causes of mortality and morbidity in the world.
Recent dramatic increases in the incidence of mosquito-borne diseases
underscore the need for new approaches to insect control based on mosquito
specific agents. The discovery of such mosquito-specific agents depends on
basicresearch on the biology of mosquitoes. However, there is a serious
deficiency in our understanding of nearly all aspects of mosquito biology.
Juvenile Hormone (JH) is amajor hormonal regulator in insects. In adult female
mosquito JH signals that ecdysis to the adult stage is complete and
reproductive processes should begin, inducing simultaneous changes in the
expression of many genes in ovarian, fat body and midgut tissues. We have shown
that JH plays an essential role in the transcriptionalactivation of the early
trypsin gene in the Aedes aegypti midgut. The early trypsin is
aremarkable"molecular marker," its expression is dramatically modified after
feeding, and it is affected by both hormones and nutrients. We propose to use
the expression of theearly trypsin gene as a paradigm for the nutritional
regulation of JET levels. The ultimate goal of this project is to understand
how changes in the nutritional status are assessed, and this information is
transduced to coordinate post-feeding physiology. In this proposal, we are
focusing on thenutritional regulation of JH levels. Our hypothesis is that the
midgut can detect the nutritional status after feeding, and regulates the JR
levels by affecting the activity of the corpora allata (CA). This regulation
could be mediated directly or indirectly bymidgut peptides synthesized by the
endocrine cells and released into the hemolymph. We have established an in
vitro radiochemical assay to measure the synthesis of JH by the CA, using this
assay we have shown that midgut peptide extracts stimulate thesynthesis of JET
by the CA, and also that an Aedes aegyptiallatotropin stimulatesthesynthesis of
JET by the CA. In order to define how JR levels are regulated after a blood
meal, the following specific aims are proposed: (1) To investigate the role of
the Aedes aegypti allatotropin and allatostatins on the regulation of CA
activity in vitro, (2) To identify identify the peptide(s) responsible for the
midgut allato-regulatory activity and (3) To investigate the physiological role
of allato-regulatory peptides "in vivo."
描述(由申请人提供):蚊子传播的寄生虫
疾病是世界上死亡和发病的主要原因之一。
最近蚊子传播疾病的发病率急剧增加
强调需要新的方法来控制昆虫,
具体的代理商。这种蚊子特异性因子的发现取决于
蚊子生物学的基础研究。然而,有一个严重的
我们对蚊子生物学的几乎所有方面都缺乏了解。
保幼激素(JH)是昆虫体内重要的激素调节剂。成年雌性
蚊子JH发出蜕皮到成虫阶段完成的信号,
生殖过程应该开始,诱导同时发生的变化
卵巢、脂肪体和中肠组织中许多基因的表达。我们已经表明
JH在早期转录激活中起着重要作用,
埃及伊蚊中肠的胰蛋白酶基因早期胰蛋白酶是
一种可标记的“分子标记”,其表达在
进食,它受到激素和营养素的影响。我们建议使用
早期胰蛋白酶基因的表达作为一个范例的营养
JET水平的调节。这个项目的最终目标是了解
如何评估营养状况的变化,这些信息是
以协调进食后的生理学。在本提案中,我们
关注JH水平的营养调节。我们的假设是
中肠可以检测摄食后的营养状况,并调节JR
通过影响咽侧体(CA)的活动水平。本法规
可以直接或间接介导的中肠肽合成的
内分泌细胞并释放到血淋巴中。我们建立了一个
体外放射化学测定,以测量CA合成JH,使用该
我们的试验表明,中肠肽提取物刺激JET的合成,
由CA,也是一种埃及伊蚊atotropin刺激合成
在CA的JET为了确定JR水平是如何调节后,血液
膳食,提出以下具体目标:(1)调查的作用,
埃及伊蚊促排素和抑排素对CA的调节作用
(2)鉴定负责体外活性的肽,
中肠的allato调节活性和(3)探讨生理作用
allato-regulatory peptides“in vivo. "
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Fernando Gabriel. Noriega其他文献
Fernando Gabriel. Noriega的其他文献
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{{ truncateString('Fernando Gabriel. Noriega', 18)}}的其他基金
How can mosquitoes develop and reproduce in the complete absence of juvenile hormone?
在完全没有保幼激素的情况下,蚊子如何发育和繁殖?
- 批准号:
10410633 - 财政年份:2022
- 资助金额:
$ 29.79万 - 项目类别:
Regulation of juvenile hormone titers in mosquitoes
蚊子保幼激素滴度的调节
- 批准号:
8097616 - 财政年份:2010
- 资助金额:
$ 29.79万 - 项目类别:
Regulation of Juvenile Hormone levels in mosquitos
蚊子保幼激素水平的调节
- 批准号:
6632099 - 财政年份:2001
- 资助金额:
$ 29.79万 - 项目类别:
Regulation of juvenile hormone titers in mosquitoes
蚊子保幼激素滴度的调节
- 批准号:
8278043 - 财政年份:2001
- 资助金额:
$ 29.79万 - 项目类别:
Regulation of juvenile hormone titers in mosquitoes
蚊子保幼激素滴度的调节
- 批准号:
7212241 - 财政年份:2001
- 资助金额:
$ 29.79万 - 项目类别:
Regulation of juvenile hormone titers in mosquitoes
蚊子保幼激素滴度的调节
- 批准号:
9090052 - 财政年份:2001
- 资助金额:
$ 29.79万 - 项目类别:
Regulation of juvenile hormone titers in mosquitoes
蚊子保幼激素滴度的调节
- 批准号:
8655511 - 财政年份:2001
- 资助金额:
$ 29.79万 - 项目类别:
Regulation of juvenile hormone titers in mosquitoes
蚊子保幼激素滴度的调节
- 批准号:
8982645 - 财政年份:2001
- 资助金额:
$ 29.79万 - 项目类别:
Regulation of Juvenile Hormone levels in mosquitos
蚊子保幼激素水平的调节
- 批准号:
6866968 - 财政年份:2001
- 资助金额:
$ 29.79万 - 项目类别:
Regulation of juvenile hormone titers in mosquitoes
蚊子保幼激素滴度的调节
- 批准号:
6984356 - 财政年份:2001
- 资助金额:
$ 29.79万 - 项目类别:
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