THE ANOPHELES GAMBIAE GENOME PROJECT

冈比亚按蚊基因组计划

• 批准号: 6262486
• 负责人: FRANK H. COLLINS
• 金额: $ 148.69万
• 依托单位: UNIVERSITY OF NOTRE DAME
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6262486
• 关键词: Anopheles; artificial chromosomes; biotechnology; complementary DNA; cooperative study; disease vectors; genetic library; genetic mapping; genome; guinea pigs; in situ hybridization; laboratory mouse; malaria; microarray technology; molecular cloning; nucleic acid sequence; polymerase chain reaction; restriction fragment length polymorphism

Malaria, tuberculosis, and AIDS are three major pathogen-specific causes of human mortality in the world. More than 90% of the world's malaria is in Africa, where the mosquito Anopheles gambiae is the primary vector. Human and malaria parasite genome projects are now underway and expected to be complete within the next year or two. This proposal seeks to initiate an A.gambiae genome project, with the expectation that the availability of the genomes of the parasite, human host, and the vector will lead to the development of novel malaria control strategies. The immediate aims of this proposal are the following: (1)to sequence 10,000 ESTs from normalized cDNA libraries made from each of five different mosquito tissues (salivary gland, midgut, fatbody, head, and immune- responsive cell lines), (2) to physically map to the mosquito's polytene chromosomes 2,000 genomic DNA Bacterial Artificial Chromosomes (BACs), (3) to sequence both ends of 25,000 genomic DNA BACs, and (4) to assess local scale synteny between the mosquito and Drosophila melanogaster by sequencing 3 megabases of sequence selected to be homologous with the well studied D. melanogaster Adh region. These 4 aims will provide sequences for direct gene discovery, for gene expression analysis (by microarray techniques), and for the determination of broad (polytene chromosome level) and local (megabase sequence level) synteny between D .melanogaster and A. gambiae. They will also facilitate a subsequent full genome sequencing project, either by a Sequence Tagged Connector strategy or by a shotgun strategy. The project is structured to involve the participation of both US and European institutions so as to make this an international effort. The long range is to encourage a broad range of funding agencies to join in supporting an effort to sequence the entire genome of this mosquito.

疟疾、结核病和艾滋病是世界上导致人类死亡的三大病原体特异性原因。 世界上90%以上的疟疾发生在非洲，冈比亚按蚊是该地区的主要媒介。人类和疟疾寄生虫基因组计划目前正在进行中，预计将在未来一两年内完成。 该提案旨在启动冈比亚疟原虫基因组项目，期望寄生虫、人类宿主和载体基因组的可用性将导致新型疟疾控制策略的开发。 该提案的直接目标如下：(1) 对来自五种不同蚊子组织（唾液腺、中肠、脂肪体、头部和免疫应答细胞系）中每一种的标准化 cDNA 文库中的 10,000 个 EST 进行测序，(2) 将 2,000 个基因组 DNA 细菌人工染色体 (BAC) 物理映射到蚊子的多线染色体，(3) 对 25,000 个基因组 DNA BAC 的两端进行测序，以及 (4) 通过对选择与充分研究的黑腹果蝇 Adh 区域同源的 3 兆碱基序列进行测序，评估蚊子和果蝇之间的局部规模同线性。 这 4 个目标将为直接基因发现、基因表达分析（通过微阵列技术）以及确定黑腹果蝇和冈比亚果蝇之间的广泛（多线染色体水平）和局部（兆碱基序列水平）同线性提供序列。 他们还将通过序列标记连接器策略或鸟枪策略促进后续的全基因组测序项目。 该项目的结构包括美国和欧洲机构的参与，以使其成为一项国际努力。 长期目标是鼓励广泛的资助机构加入支持对这种蚊子的整个基因组进行测序的努力。