MALARIA CONTROL BY GENETIC MANIPULATION OF VECTORS

通过载体基因调控控制疟疾

• 批准号: 6362408
• 负责人: FRANK H. COLLINS
• 金额: $ 67.02万
• 依托单位: UNIVERSITY OF NOTRE DAME
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6362408
• 关键词: Anopheles; disease vectors; genetically modified animals; malaria; transfection

The broad aim of this Project Proposal is to develop the tools that can form the basis of a genetic control strategy for malaria in sub-Saharan African transmitted by the vector Anopheles gambiae. This TDRU Program Project involves 4 specific projects. The specific aims of Project 1 are the development of two different techniques for germ lines transformation of An. Gambiae, a transposon-based method and a site- specific recombinase method for introducing larger fragments of DNA into the genome. The aim of project 2 is to develop transgene constructs that can block transmission blocking antibody and another based on the small, antiparasitic peptides like cecropins and megainins. The aim of project 3 is to develop and test antiparasite transgene constructs based on An. Gambiae immune peptides that will be delivered to the hemocoel. The goal of Project 4 is to test the hypothesis that a meiotic drive system can be used as the necessary tool for driving a genetically engineered parasite refractory genotype (such as those produced in Projects 2 and 3) into the wild population.

本项目建议书的广泛目标是开发工具，为撒哈拉以南非洲地区由冈比亚按蚊传播的疟疾的遗传控制战略奠定基础。本TDRU计划项目涉及4个具体项目。项目1的具体目标是开发两种不同的技术，用于AN的生殖系转化。Gambiae，一种基于转座子的方法和一种用于将较大DNA片段引入基因组的位点特异性重组酶方法。项目2的目的是开发转基因结构，可以阻断传输阻断抗体和另一个基于小的抗寄生虫肽，如天蚕素和巨霉素。项目3的目的是开发和测试抗寄生虫转基因构建体的基础上，一个。冈比亚免疫肽将被输送到血腔。项目4的目标是检验以下假设：减数分裂驱动系统可用作将基因工程寄生虫抗性基因型（如项目2和3中产生的基因型）驱动到野生种群中的必要工具。