Neuropeptidase Function

神经肽酶功能

• 批准号: 6395239
• 负责人: David W Rodgers
• 金额: $ 27.84万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-15 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6395239
• 关键词: X ray crystallography; chemical kinetics; computer simulation; conformation; crystallization; enzyme induction /repression; enzyme inhibitors; enzyme model; enzyme substrate analog; enzyme substrate complex; intermolecular interaction; metalloendopeptidases; model design /development; molecular site; molecular size; neuropeptides; neuroregulation; neurotensin; peptide chemical synthesis; peptide library; physical model; protein folding; protein purification; protein structure function; site directed mutagenesis; structural biology

Neuropeptides are a diverse assembly of small peptides that serve as neurotransmitters and neuromodulators in the central nervous system and as neurohormones in the periphery. The levels of these signaling molecules are known to be modulated by many centrally active agents and affected in a number of disease states-including psychotic disorders, Alzheimer's disease, and hypertension. Neuropeptides are inactivated by a group of proteolytic enzymes known as neuropeptidases. Therapeutic modulation of these enzymes can form the basis of effective treatments by altering the levels of their neuropeptide substrates, and they are therefore important drug targets. These efforts are hampered, however, both because substrate specificity is not understood and because there is a little structural information on the binding of substrates and inhibitors, which could be used for improving therapeutics. The goal of the proposed study is an understanding of specificity and function in neuropeptidases using endopeptidase 24.16 (neurolysin) as a model system. This enzyme, a zinc metallopeptidase, inactivates the neuropeptide neurotensin and may also participate in the inactivation of other neuroactive peptides. Endopeptidase 24.16 has two unusual properties shared by a number of neuropeptidases: it cleaves only short oligopeptides, and it recognizes a highly diverse set of cleavage sites. The basis for these unusual properties will be explored by an approach combining high- resolution structure determination and functional studies of the enzyme. We have determined the crystal structure of endopeptidase 24.16, which shows a deep active channel that prevents access by proteins. We will test the hypotheses that the length of substrates is further limited by the nature of the active site channel and that diverse sequences are accommodated by a high degree of plasticity in the catalytic region. Four specific aims are proposed: 1) to determine crystal structures with a series of peptides and inhibitors that will reveal the details of substrate binding, 2) to characterize the functional aspects of specificity through systematic variation of substrate features, 3) to identify important features of recognition by characterizing a closely related endopeptidase, 4) to test and refine our models of recognition by modulating and altering the specificity of the enzyme through mutagenesis. This work will define the general mechanisms of neuropeptide inactivation and provide a detailed view of inhibitor binding, laying the foundation for future therapy development.

神经肽是小分子肽的多样性集合，其在中枢神经系统中充当神经递质和神经调质，在外周中充当神经激素。已知这些信号分子的水平受到许多中枢活性剂的调节，并在许多疾病状态中受到影响，包括精神障碍、阿尔茨海默病和高血压。 神经肽被称为神经肽酶的一组蛋白水解酶灭活。这些酶的治疗调节可以通过改变其神经肽底物的水平来形成有效治疗的基础，因此它们是重要的药物靶标。 然而，这些努力受到阻碍，因为底物特异性不被理解，并且因为底物和抑制剂结合的结构信息很少，这可以用于改善治疗。 拟议的研究的目标是了解的特异性和功能的神经肽酶使用内肽酶24.16（神经溶解素）作为模型系统。 这种酶是一种锌金属肽酶，可使神经肽神经降压素失活，也可能参与其他神经活性肽的失活。 内肽酶24.16有两个不寻常的特性，许多神经肽酶共享：它只切割短的寡肽，它识别一组高度多样化的切割位点。 这些不寻常的性质的基础将探索的方法相结合的高分辨率结构测定和功能研究的酶。 我们已经确定了内肽酶24.16的晶体结构，它显示了一个阻止蛋白质进入的深活性通道。 我们将测试的假设，即基板的长度进一步限制的性质的活性位点通道和不同的序列被容纳在催化区域的高度可塑性。 提出了四个具体目标：1）用一系列肽和抑制剂确定晶体结构，这些肽和抑制剂将揭示底物结合的细节，2）通过底物特征的系统变化来表征特异性的功能方面，3）通过表征密切相关的内肽酶来鉴定识别的重要特征，4）通过诱变调节和改变酶的特异性来测试和改进我们的识别模型。 这项工作将定义神经肽失活的一般机制，并提供抑制剂结合的详细视图，为未来的治疗发展奠定基础。