PROTEIN ANALYTICAL CORE

蛋白质分析核心

• 批准号: 8168245
• 负责人: David W Rodgers
• 金额: $ 9.9万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168245
• 关键词: Bioreactors; Calorimetry; Cells; Centers of Research Excellence; Chromatography; Computer Retrieval of Information on Scientific Projects Database; Equipment; Fluorescence; Funding; Grant; High Pressure Liquid Chromatography; Human Resources; Individual; Institution; Laboratories; Production; Proteins; Recombinant Proteins; Research; Research Personnel; Resources; Schedule; Services; Source; Titrations; Total Internal Reflection Fluorescent; Training; United States National Institutes of Health; X-Ray Crystallography; analytical ultracentrifugation; base; cost; design; instrumentation; light scattering; macromolecule; member; single molecule; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Protein Analytical Core is designed to facilitate recombinant protein production and macromolecular characterization by the COBRE investigators, key components of their proposed projects. It makes available well-maintained equipment and instrumentation that would not normally be found within individual laboratories, particularly those of junior investigators. The approach is to provide training and priority access to members of the investigators'' teams rather than maintaining the personnel required to accomplish the tasks on a service basis. In this way, costs are kept to a minimum. The core provides instrumentation for characterizing macromolecules, including dynamic light scattering, spectropolarimetry, fluorescence, isothermal titration calorimetry, high performance liquid chromatography, X-ray crystallography, TIRF microscopy for single molecule studies, and analytical ultracentrifugation. In addition, fermenters, bioreactors, tissue culture facilities, cell cracking equipment, and chromatography instrumentation are available for production and purification of recombinant proteins. A staff member maintains the core equipment, provides training to new users, and oversees scheduling.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 蛋白质分析核心旨在促进COBRE研究人员的重组蛋白生产和大分子表征，这是他们拟议项目的关键组成部分。它提供了维护良好的设备和仪器，这些设备和仪器通常不会在个别实验室内找到，特别是初级调查人员的设备和仪器。采取的办法是为调解员小组成员提供培训和优先准入，而不是维持完成任务所需的人员。通过这种方式，成本被保持在最低限度。核心提供表征大分子的仪器，包括动态光散射、分光偏振、荧光、等温滴定量热、高效液相色谱、X射线晶体学、用于单分子研究的TIRF显微镜和分析超离心。此外，发酵罐、生物反应器、组织培养设施、细胞裂解设备和色谱仪器可用于生产和纯化重组蛋白。一名工作人员维护核心设备，为新用户提供培训，并监督调度。