PROTEIN ANALYTICAL CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 蛋白质分析核心旨在促进Cobre研究人员的重组蛋白质生产和大分子表征，这是他们拟议项目的关键组成部分。它提供了维护良好的设备和仪器，这些设备和仪器通常不会在个别实验室内找到，特别是初级调查人员的实验室。办法是向调查组成员提供培训和优先准入，而不是保留完成任务所需的服务人员。通过这种方式，成本被保持在最低限度。该核心提供表征大分子的仪器，包括动态光散射、光谱偏振、荧光、等温滴定量热法、高效液相色谱、X射线结晶学、用于单分子研究的TIRF显微镜和分析性超速离心法。此外，发酵罐、生物反应器、组织培养设施、细胞裂解设备和层析仪器可用于生产和纯化重组蛋白。一名工作人员维护核心设备，为新用户提供培训，并监督日程安排。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Protein Analytical Core is designed to facilitate recombinant protein production and macromolecular characterization by the COBRE investigators, key components of their proposed projects. It makes available well-maintained equipment and instrumentation that would not normally be found within individual laboratories, particularly those of junior investigators. The approach is to provide training and priority access to members of the investigators'' teams rather than maintaining the personnel required to accomplish the tasks on a service basis. In this way, costs are kept to a minimum. The core provides instrumentation for characterizing macromolecules, including dynamic light scattering, spectropolarimetry, fluorescence, isothermal titration calorimetry, high performance liquid chromatography, X-ray crystallography, TIRF microscopy for single molecule studies, and analytical ultracentrifugation. In addition, fermenters, bioreactors, tissue culture facilities, cell cracking equipment, and chromatography instrumentation are available for production and purification of recombinant proteins. A staff member maintains the core equipment, provides training to new users, and oversees scheduling.