CORE--TISSUE CULTURE & PROTEIN PRODUCTION

核心——组织培养

• 批准号: 6972208
• 负责人: David W Rodgers
• 金额: $ 15.77万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6972208
• 关键词: biomedical equipment; biomedical facility; protein biosynthesis; recombinant proteins; tissue /cell culture

The Tissue Culture and Protein Production Core is designed to facilitate cell culture and recombinant protein production by the COBRE investigators, key components of their proposed projects. It will make available well-maintained equipment and instrumentation that would not normally be found within individual laboratories, particularly those of junior investigators. The approach is to provide training and priority access to members of the investigators' teams rather than maintaining the personnel required to accomplish the tasks on a service basis. In this way, costs will be kept to a minimum. The Core will provide tissue culture hoods and incubators for mammalian cell culture. In addition, fermenters, bioreactors, cell cracking equipment, and chromatography instrumentation will be available for production of recombinant proteins. Instrumentation to characterize recombinant proteins will also be available in the Core. A single staff member will maintain the Core equipment, provide training to new users, and oversee scheduling.

组织培养和蛋白质生产核心旨在促进细胞培养， 重组蛋白生产的COBRE研究人员，他们提出的关键组成部分， 项目它将提供维护良好的设备和仪器， 通常在个别实验室内发现，特别是初级研究人员的实验室。的 办法是向调查小组成员提供培训和优先接触机会， 而不是在服务的基础上维持完成任务所需的人员。通过这种方式， 费用将保持在最低限度。 核心将提供用于哺乳动物细胞培养的组织培养罩和培养箱。此外，本发明还提供了一种方法， 发酵罐、生物反应器、细胞裂解设备和色谱仪器将被 可用于生产重组蛋白。表征重组体的仪器 蛋白质也将在核心中可用。 一名工作人员将维护核心设备，为新用户提供培训，并监督调度。