KY COBRE: TISSUE CULTURE & PROTEIN PRODUCTION CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 组织培养和蛋白质生产核心旨在促进Cobre研究人员的细胞培养和重组蛋白质生产，这是他们拟议项目的关键组成部分。它将提供维护良好的设备和仪器，这些设备和仪器通常不会在个别实验室内找到，特别是初级调查人员的实验室。办法是向调查组成员提供培训和优先准入，而不是保留完成任务所需的服务人员。通过这种方式，成本将保持在最低水平。 Core将为哺乳动物细胞培养提供组织培养罩和孵化器。此外，发酵罐、生物反应器、细胞裂解设备和层析仪器将可用于生产重组蛋白质。Core还将提供表征重组蛋白质的仪器。 一名工作人员将维护核心设备，为新用户提供培训，并监督日程安排。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Tissue Culture and Protein Production Core is designed to facilitate cell culture and recombinant protein production by the COBRE investigators, key components of their proposed projects. It will make available well-maintained equipment and instrumentation that would not normally be found within individual laboratories, particularly those of junior investigators. The approach is to provide training and priority access to members of the investigators'' teams rather than maintaining the personnel required to accomplish the tasks on a service basis. In this way, costs will be kept to a minimum. The Core will provide tissue culture hoods and incubators for mammalian cell culture. In addition, fermenters, bioreactors, cell cracking equipment, and chromatography instrumentation will be available for production of recombinant proteins. Instrumentation to characterize recombinant proteins will also be available in the Core. A single staff member will maintain the Core equipment, provide training to new users, and oversee scheduling.