PROTEIN ANALYTICAL CORE

蛋白质分析核心

• 批准号: 8360571
• 负责人: David W Rodgers
• 金额: $ 8.09万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360571
• 关键词: Bioreactors; Calorimetry; Cells; Centers of Research Excellence; Chromatography; Equipment; Fluorescence; Funding; Grant; High Pressure Liquid Chromatography; Human Resources; Individual; Laboratories; Molecular; National Center for Research Resources; Principal Investigator; Production; Proteins; Recombinant Proteins; Research; Research Infrastructure; Research Personnel; Resources; Schedule; Services; Source; Titrations; Total Internal Reflection Fluorescent; Training; United States National Institutes of Health; X-Ray Crystallography; analytical ultracentrifugation; base; cost; design; human disease; instrumentation; light scattering; macromolecule; member; single molecule; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Protein Analytical Core is designed to facilitate recombinant protein production and macromolecular characterization by the COBRE investigators, key components of their proposed projects. It makes available well-maintained equipment and instrumentation that would not normally be found within individual laboratories, particularly those of junior investigators. The approach is to provide training and priority access to members of the investigators'' teams rather than maintaining the personnel required to accomplish the tasks on a service basis. In this way, costs are kept to a minimum. The core provides instrumentation for characterizing macromolecules, including dynamic light scattering, spectropolarimetry, fluorescence, isothermal titration calorimetry, high performance liquid chromatography, X-ray crystallography, TIRF microscopy for single molecule studies, and analytical ultracentrifugation. In addition, fermenters, bioreactors, tissue culture facilities, cell cracking equipment, and chromatography instrumentation are available for production and purification of recombinant proteins. A staff member maintains the core equipment, provides training to new users, and oversees scheduling.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 蛋白质分析核心旨在促进Cobre研究人员的重组蛋白质生产和大分子表征，这是他们拟议项目的关键组成部分。它提供了维护良好的设备和仪器，这些设备和仪器通常不会在个别实验室内找到，特别是初级调查人员的实验室。办法是向调查组成员提供培训和优先准入，而不是保留完成任务所需的服务人员。通过这种方式，成本被保持在最低限度。该核心提供表征大分子的仪器，包括动态光散射、光谱偏振、荧光、等温滴定量热法、高效液相色谱、X射线结晶学、用于单分子研究的TIRF显微镜和分析性超速离心法。此外，发酵罐、生物反应器、组织培养设施、细胞裂解设备和层析仪器可用于生产和纯化重组蛋白。一名工作人员维护核心设备，为新用户提供培训，并监督日程安排。