NEUROENDOCRINE & GONADAL CONTROL OF MALE REPRODUCTION

描述：(根据申请者的描述改编)这份哈佛生殖专业中心的申请书建议在男性身上引入神经内分泌和性腺对生殖的控制。其中两个是生殖的组成部分，另外两个将探讨性腺水平作为间质细胞对类固醇生成的控制。在项目I中，将在特发性低促性腺激素减退症(IHH)患者中使用串联表型和基因分型方法。这是一个人类模型，其中一系列基因缺陷导致性成熟的神经内分泌部分失败。在项目II中，Ursula Kaiser博士将使用起源于促性腺激素的黄体生成素-T2细胞探索促性腺激素基因的GnRH。通过使用细胞色素P17基因和类固醇激素受体抑制类固醇激素的合成。此外，她还将绘制这种受体的发育表达图，并将其与类固醇的生成联系起来。在项目IV中，Joel Habener博士将继续探索cAMP激活的转录因子CREB编码基因选择性剪接途径的作用。他还将探索垂体腺苷环化酶激活肽(PACAP)及其选择性剪接的1型受体在精子发生的自分泌控制中的作用。

DESCRIPTION: (Adapted from the applicant's description) This application for the Harvard-wide Specialized Center for Reproduction proposes to bring the neuroendocrine and gonadal control of reproduction in the male. Two of the component of reproduction while the other two will explore the gonadal level as Leydig cell control of steroidogenesis. In Project I, will employ tandem phenotyping and genotyping approaches in patients with idiopathic hypogonadotropic hypogonadism (IHH). This is a human model in which a series of genetic defects result in failure of the neuroendocrine component of sexual maturation. In Project II, Dr. Ursula Kaiser will explore the GnRH of gonadotropic genes using LH-T2 cells which are of gonadotrope origin. In Inhibiting Substance (MIS) upon steroidogenesis using the CYP17 gene as well MIS receptor. In addition, she will chart the developmental expression of this receptor and correlate it with steroidogenesis. In Project IV, Dr. Joel Habener will continue his explorations of the role of cAMP-activated pathways of alternative splicing of the gene encoding of the transcription factor, CREB. He will also explore the role of pituitary adenyl cyclase activating peptide; PACAP, and its alternatively spliced type 1 receptor in the autocrine control of spermatogenesis.