PEPTIDE SYNTHESIS, EVALUATION AND PUFIFICATION SYSTEM

肽合成、评价和纯化系统

• 批准号: 6054059
• 负责人: FRANCES M FINN
• 金额: $ 19.06万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-14 至 2001-04-13
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6054059
• 关键词: biomedical equipment purchase; high performance liquid chromatography; peptide chemical synthesis

This request is for funds to purchase an Advanced ChemTech 396 dual robotic peptide synthesis instrument for multiple, simultaneous peptide synthesis and Waters Alliance HPLC equipment to evaluate the purity of the peptides produced and to purify them as required. These instruments are an operationally integrated system, essential for the production of synthetic peptides in the Peptide Synthesis Facility, a core facility. The need for these instruments is justified by 1) a significantly increased demand for synthetic peptides by University of Pittsburgh Cancer Institute NIH-supported investigators; 2) plans to expand the area served by the Facility to include NIH-funded investigators from the entire University of Pittsburgh community; and 3) the age and lack of cost effectiveness of the present instruments (an eleven year old Applied Biosystems 430A and Waters HPLC equipment). Shortage of synthetic capacity has been a source of frustration to many of the Facility users because of the long delays experienced between placing an order and receiving the finished product This has resulted in the need to order peptides outside the Faculty despite the high quality of the peptides produced here. At present, members of the University of Pittsburgh Cancer Institute (UPCI) constitute the major users for the Facility. The importance of the Peptide Synthesis Facility to the broader NIH-funded community is recognized by institutional support for the Facility.

该申请是为了获得资金，用于购买Advanced ChemTech 396双机器人肽合成仪器，用于多个同步肽合成，以及沃茨Alliance HPLC设备，用于评估所生产肽的纯度并根据需要对其进行纯化。这些仪器是一个操作集成系统，对于肽合成设施（核心设施）中合成肽的生产至关重要。对这些仪器的需求是合理的：1）匹兹堡大学癌症研究所NIH支持的研究人员对合成肽的需求显着增加; 2）计划扩大该设施服务的区域，以包括来自整个匹兹堡大学社区的NIH资助的研究人员;和3）现有仪器（十一年前的AppliedBiosystems 430 A和沃茨HPLC设备）的老化和缺乏成本效益。合成能力的短缺一直是令许多设施用户感到沮丧的原因，因为在下订单和收到成品之间经历了很长时间的延迟。这导致了尽管这里生产的肽质量很高，但仍需要在学院外订购肽。目前，匹兹堡大学癌症研究所的成员是该基金的主要用户。肽合成设施的重要性，以更广泛的NIH资助的社区是公认的机构支持的设施。