CELL HARVESTER & MULTILABEL COUNTER

用于分析细胞增殖、酶功能、凋亡、细胞-细胞相互作用和受体-配体研究的自动化设备的发展极大地促进了这些领域的研究。Wallac microbetatplate Trilux收割机和Victor多标签计数器是测量放射性、化学发光、荧光和光度测定的集成系统的组成部分。该设备允许以自动化方式分析大量样品，并可用于标记分子的定量测量。它的通用性将大大促进MH支持研究的进展。由于购买该仪器所涉及的主要成本，必须考虑共享使用。本提案概述了六个主要用户的研究计划，并为其购买申请资金。美国国立卫生研究院资助的研究概述了一系列广泛的技术，这些技术都将从使用该设备中受益匪浅。这六个主要用户都是钱宁实验室的成员，并且有着强大的合作和科学互动的历史。该仪器的共享使用将体现钱宁实验室的模式，即集中资源，加强其成员的整体研究工作。拟议文书的使用应有助于加强这一努力。总而言之，本提案请求资金用于购买Wallac microbetatplate Trilux收割机和Victor多标签计数器，这将由六个主要用户共享。包括钱宁实验室主任在内的这些用户已作出重大承诺，提供维护和操作该仪器所需的财政支助。仪器的可用性将大大加快MH资助的研究概述。

The development of automated equipment for the analysis of cellular proliferation, enzyme function, apoptosis, cell-cell interactions, and receptor-ligand studies has greatly facilitated research in these areas. The Wallac Microbetaplate Trilux harvester and Victor multilabel counter are components of an integrated system that measures radioactivity, chemiluminescence, fluorescence, and photometry. This equipment allows for analysis of a large number of samples in an automated fashion and can be used for quantitative measurement of labeled molecules. Its versatility should greatly enhance the progress of MH supported research. Due to the major cost involved in its purchase, the instrument must be considered for shared-use. This proposal outlines the Research Plans of six major users and requests funding for its purchase. The NIH funded research outlined encompass a broad range of technologies that will all greatly benefit from use of this equipment. The six major users are all members of the Channing Laboratory and have a strong history of collaboration and scientific interaction. The shared use of this instrument will exemplify the model of the Channing Laboratory in which resources are pooled to strengthen the overall research effort of its members. The use of the proposed instrument should serve to fortify this effort. In summary, this proposal requests funds for the purchase of a Wallac Microbetaplate Trilux harvester and Victor multilabel counter that will be shared by six major users. A major commitment has been made by these users, including the Director of the Channing Laboratory, to provide the financial support required to maintain and operate the instrument. The availability of the instrument will greatly accelerate the MH funded research outlined.