Innovative RNA Profiling in Cancer Cells

癌细胞中创新的 RNA 分析

• 批准号: 6401352
• 负责人: Jiwu Wang
• 金额: $ 14.95万
• 依托单位: ALLELE BIOTECHNOLOGY AND PHARMACEUTICALS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2002-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6401352
• 关键词: RNA; RNA splicing; gene expression; high throughput technology; human genetic material tag; neoplasm /cancer genetics; neoplastic cell; nucleic acid amplification techniques; nucleic acid sequence; technology /technique development

The ultimate goal of this proposal is to develop and commercialize a novel and powerful assay system for amplification and detection of gene transcripts in cancer-related studies. Specifically, our proprietary technology is targeted to providing tools for two types of applications. First, we will develop an assay system that can be used for in vitro RNA amplification. It will provide effective tools for studies of gene expression in cancer cells. It can also be used for detecting infectious agents in cancer samples. Second, we will test the feasibility of using our techniques for detection of gene expression in solid cancer specimens. Thus spatial distribution of expressed transcripts can be monitored directly on these samples. We also plan to devote our efforts to integrate our system into a technology platform that is compatible with modern techniques such as microarray, nanoparticle detection, and optical scanning. Molecular profiling of cancer with this technology will enable systematic analysis of perturbations of transcription initiation, alternative splicing, or RNA stability. The entire system is expected to have wide potential for applications in basic, preclinical and clinical cancer research. PROPOSED COMMERCIAL APPLICATIONS: The proposed technology will be developed into marketable products such as lab kits or field kits for gene expression detection. Other application formats can be build upon the technology platform. Particularly, we will cooperate with other companies to develop analysis systems for high-throughput screening of cancer-related alterations of gene expression. The profitability of these systems is expected to be very high because of their enormous potential in biomedical field.

该提案的最终目标是开发和商业化一种新的和强大的分析系统，用于扩增和检测癌症相关研究中的基因转录本。具体而言，我们的专有技术旨在为两种类型的应用程序提供工具。首先，我们将开发一种可用于体外RNA扩增的测定系统。它将为研究癌细胞基因表达提供有效的工具。它也可用于检测癌症样品中的感染因子。其次，我们将测试使用我们的技术检测实体癌标本中基因表达的可行性。因此，可以直接在这些样品上监测表达的转录物的空间分布。我们还计划致力于将我们的系统整合到一个与现代技术（如微阵列，纳米颗粒检测和光学扫描）兼容的技术平台中。用这种技术进行癌症的分子谱分析将能够系统地分析转录起始、可变剪接或RNA稳定性的扰动。整个系统有望在基础、临床前和临床癌症研究中具有广泛的应用潜力。拟议的商业应用：该技术将被开发成可销售的产品，如用于基因表达检测的实验室试剂盒或现场试剂盒。其他应用程序格式可以建立在技术平台上。特别是，我们将与其他公司合作开发用于高通量筛选癌症相关基因表达改变的分析系统。由于这些系统在生物医学领域的巨大潜力，其盈利能力预计将非常高。