SBIR Topic 290 ^ ^siRNA Resource for Synthetic Lethal Screening of DNA Repair and

本提案的目的是开发基因沉默试剂，并测试/验证其在以下方面的用途： DNA损伤信号传导和修复途径的合成致死筛选。使这些试剂可用将提供效率和方便，癌症研究人员目前需要找到新的策略和治疗癌症。 DNA修复途径基因已被证明编码癌症生物发生中的重要因素。靶向该途径中的不同网络可能是治疗癌症的一个有希望的行动点。拟议的研究可以促进寻找这种治疗方法的努力。该研究计划包括一系列重要元素的新组合，例如，高效构建携带shRNA的慢病毒，以及使用替代靶点进行基因沉默试验。

The objectives of this proposal are to develop gene silencing reagents, and to test/validate their use in synthetic lethal screening of DNA damage signaling and repair pathways. Making such reagents available will provide the efficiency and convenience that cancer researchers currently need to find novel strategies and therapeutics against cancer. DNA repair pathway genes have been shown to encode important factors in cancer biogenesis. Targeting different networks within this pathway may be a promising point of action for curing cancer. The proposed research can facilitate the efforts in finding such cures. The research plan includes a novel combination of a number of essential elements, for example, highly efficient construction of lentiviruses carrying shRNA and use of surrogate targets for gene silencing assays.