Novel Technology for Field Detection of Gene Expression

描述(由申请人提供)： 这项提议的总体目标是开发一种创新的 基因表达谱技术。这项专利技术是 基于RNA聚合酶直接扩增RNA。高效的协议 将建立扩增和检测RNA、mRNAs的方法 特别适用于现场应用。这部小说 技术应该在效率和易用性方面提供巨大的改进 与目前可用的方法，例如RT-PCR相比，使用。使用 RNA样品的提取、保存及最新研究进展 光信号检测，这项技术也将使高度敏感 以及对基因表达变化的高通量评估以响应 暴露在环境中。该系统的可行性将首先得到论证 在拟议的研究中。然后将开发产品，以提供易于 使用廉价和便利的工具来识别扰动 对毒物暴露作出反应的基因表达模式。备择 将利用协议来建立最佳技术。这个 可以格式化用于微阵列的方法。该系统预计将具有 环境研究和流行病学调查的广泛潜在用途，因为我们 就像在生物医学研究和开发的其他领域一样。 建议的商业应用： 他人开发的检测方法、检测试剂盒和检测系统组件。

DESCRIPTION (provided by applicant): The general aim of this proposal is to develop and commercialize an innovative technology for gene expression profiling. This proprietary technology is based on direct amplification of RNA by RNA polymerase. Efficient protocols will be established for amplification and detection of RNA, mRNAs in particular, that will be suitable for in field applications. This novel technology should provide great improvements in both efficiency and ease of use as compared to the methods currently available, for example, RT-PCR. With the recent advances in RNA sample extraction and preservation as well as optical signal detection, this technology will also enable highly sensitive and high-throughput assessment of changes to gene expression in response to environmental exposure. Feasibility of this system will be first demonstrated in the proposed studies. Products will then be developed to provide easy-to- use, inexpensive and expedient tools for the identification of perturbations of gene expression patterns in response to toxic exposure. Alternative protocols will be exploited in order to establish the optimum techniques. The methodology may be formatted for microarray. This system is expected to have wide potential use for environmental studies and epidemiology surveys, as we as in other fields of biomedical research and development. PROPOSED COMMERCIAL APPLICATION: Detection methods, detection kits, and components of detection system developed by others.