Advanced Platform of RNAi Technology

RNAi技术先进平台

• 批准号: 6702393
• 负责人: Jiwu Wang
• 金额: $ 9.95万
• 依托单位: ALLELE BIOTECHNOLOGY AND PHARMACEUTICALS
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6702393
• 关键词: DNA binding protein; DNA directed RNA polymerase; RNA; biotechnology; cell line; chemical conjugate; crosslink; double stranded RNA; gene delivery system; gene induction /repression; genetic promoter element; genetic techniques; genetic transduction; oligonucleotides; peptide chemical synthesis; polymerase chain reaction; reagent /indicator; technology /technique development; transfection; transport proteins

RNA interference (RNAi) is a powerful tool for gene function studies and drug development. Currently, application of this technology is largely limited by the efficiency of delivering RNAi-inducing reagents into mammalian cells. The aim of this proposal is to establish techniques to efficiently transfect siRNA-expressing cassettes. We have already developed a system to induce R NAi effects in mammalian I cells using novel DNA-based expression cassettes that offer gr eat advantages over other RNA or plasmid-based methods. We plan to take advantage of the unique design of our existing core technology by using transducing peptides as dell very vehicles. This will be particularly useful for RNAi-based gene silencing in traditionally hard to transfect cells such as primary cultures. T hese techniques can be further integrated into novel platforms for high throughput discovery and adapted for use in live animals. Development of these methods may also lay the foundations for exciting applications of our RNAi technology as therapeutic agents.

RNA干扰（RNAi）是用于基因功能研究和药物开发的强大工具。当前，该技术的应用在很大程度上受到将RNAi诱导试剂诱导到哺乳动物细胞的效率的限制。该提案的目的是建立有效转染siRNA表达盒式盒式磁带的技术。我们已经开发了一种系统，使用新型的基于DNA的表达盒在哺乳动物I细胞中诱导R NAI效应，该盒具有比其他基于RNA或基于质粒的方法的饮食优势。我们计划通过将转导肽作为戴尔非常车辆来利用现有核心技术的独特设计。这对于基于RNAi的基因沉默在传统上很难转染细胞（例如原发性培养物）特别有用。可以将技术技术进一步整合到新的平台中，以进行高吞吐量发现，并适用于活动物。这些方法的开发也可能为我们的RNAi技术的令人兴奋的应用奠定基础 作为治疗剂。