EFFICIENT CELL-FREE SELECTION OF ANTIBODIES

描述：本提案的目的是开发一种新的无细胞 该系统将有助于快速选择的功能片段， 克隆抗体所述系统基于完善的 分子机制对于抗体选择，使用编码抗体的cDNA文库。 单链可变区片段（scFv）可用于体外免疫反应， 产生可以用靶选择的大的scFv库的系统 抗原可以回收所需scFv的cDNA并用于表达 大量的重组scFv。这个过程更有效，更少 昂贵且技术上比所有现有的抗体选择方法更容易。 它也有可能适用于高通量筛选系统，或 修饰以筛选抗体以外的结合蛋白。 拟定商业应用： 第一阶段研究的目标是测试这项创新技术的可行性。 一旦开发出来，它可能对蛋白质组学、癌症诊断和药物治疗产生重大影响。 发展

DESCRIPTION: The objective of this proposal is to develop a novel cell-free system that would facilitate rapid selection of functional fragments of monoclonal antibodies. The system descibed is based on well-established molecular mechanisms. For antibody selection, a cDNA library encoding the single-chain variable region fragment (scFv) can be used in an in vitro system to generate a large pool of scFv's that can be selected with target antigens. The cDNA for the desired scFv can be recovered and used to express large quantities of recombinant scFv. This procedure is more efficient, less expensive and technically easier than all existing methods for antibody selection. It also has the potential to be adapted to high throughput screening systems, or modified to screen binding proteins other than antibodies. PROPOSED COMMERCIAL APPLICATION: The goal for the phase I studies is to test the feasibility of this innovative technology. Once developed, it may have a major impact on proteomics, cancer diagnosis and drug development.