EFFICIENT CELL-FREE SELECTION OF ANTIBODIES

描述：这项提议的目标是开发一种新型的无细胞 系统将有助于快速选择功能片段 单克隆抗体。所描述的系统是基于完善的 分子机制。为了进行抗体选择，构建了一个编码 单链可变区片段(ScFv)可用于体外 系统以生成可与目标一起选择的大量scFv池 抗原。可以回收所需单链抗体的cDNA并用于表达 大量表达重组单链抗体。这一过程更有效率，更少 与所有现有的抗体选择方法相比，该方法价格昂贵，技术上更容易。 它还具有适应高通量筛选系统的潜力，或者 经修饰以筛选抗体以外的结合蛋白。 建议的商业应用： 第一阶段研究的目标是测试这项创新技术的可行性。 一旦开发出来，它可能会对蛋白质组学、癌症诊断和药物产生重大影响 发展。

DESCRIPTION: The objective of this proposal is to develop a novel cell-free system that would facilitate rapid selection of functional fragments of monoclonal antibodies. The system descibed is based on well-established molecular mechanisms. For antibody selection, a cDNA library encoding the single-chain variable region fragment (scFv) can be used in an in vitro system to generate a large pool of scFv's that can be selected with target antigens. The cDNA for the desired scFv can be recovered and used to express large quantities of recombinant scFv. This procedure is more efficient, less expensive and technically easier than all existing methods for antibody selection. It also has the potential to be adapted to high throughput screening systems, or modified to screen binding proteins other than antibodies. PROPOSED COMMERCIAL APPLICATION: The goal for the phase I studies is to test the feasibility of this innovative technology. Once developed, it may have a major impact on proteomics, cancer diagnosis and drug development.