Novel Technology for Field Detection of Gene Expression

基因表达现场检测新技术

• 批准号: 6518245
• 负责人: Jiwu Wang
• 金额: $ 19.84万
• 依托单位: ALLELE BIOTECHNOLOGY AND PHARMACEUTICALS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2003-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6518245
• 关键词: environmental contamination; environmental exposure; field study; gene expression; high throughput technology; nucleic acid amplification techniques; optics; technology /technique development; toxicant screening; toxicology; toxin

DESCRIPTION (provided by applicant): The general aim of this proposal is to develop and commercialize an innovative technology for gene expression profiling. This proprietary technology is based on direct amplification of RNA by RNA polymerase. Efficient protocols will be established for amplification and detection of RNA, mRNAs in particular, that will be suitable for in field applications. This novel technology should provide great improvements in both efficiency and ease of use as compared to the methods currently available, for example, RT-PCR. With the recent advances in RNA sample extraction and preservation as well as optical signal detection, this technology will also enable highly sensitive and high-throughput assessment of changes to gene expression in response to environmental exposure. Feasibility of this system will be first demonstrated in the proposed studies. Products will then be developed to provide easy-to- use, inexpensive and expedient tools for the identification of perturbations of gene expression patterns in response to toxic exposure. Alternative protocols will be exploited in order to establish the optimum techniques. The methodology may be formatted for microarray. This system is expected to have wide potential use for environmental studies and epidemiology surveys, as we as in other fields of biomedical research and development. PROPOSED COMMERCIAL APPLICATION: Detection methods, detection kits, and components of detection system developed by others.

描述（由申请人提供）： 该提案的一般目的是开发和商业化创新的 用于基因表达分析的技术。 这种专有技术是 基于RNA聚合酶直接扩增RNA。 有效的协议 将建立用于放大和检测RNA，mRNA中的mRNA 特别是适合在现场应用程序中。 这本小说 技术应在效率和便利性方面提供极大的提高 与当前可用的方法相比，使用RT-PCR。 和 RNA样品提取和保存以及 光信号检测，该技术还将启用高度敏感的 以及对基因表达变化的高通量评估 环境暴露。 该系统的可行性将首先证明 在拟议的研究中。 然后将开发产品以提供易于 - 使用，便宜且方便的工具来识别扰动 响应毒性暴露的基因表达模式。 选择 将利用协议以建立最佳技术。 这 方法可以为微阵列格式化。 预计该系统将有 作为我们的环境研究和流行病学调查的广泛潜在用途 与其他生物医学研发领域一样。 拟议的商业应用： 其他人开发的检测方法，检测套件和检测系统的组件。