Novel Technology for Field Detection of Gene Expression

基因表达现场检测新技术

• 批准号: 6518245
• 负责人: Jiwu Wang
• 金额: $ 19.84万
• 依托单位: ALLELE BIOTECHNOLOGY AND PHARMACEUTICALS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2003-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6518245
• 关键词: environmental contamination; environmental exposure; field study; gene expression; high throughput technology; nucleic acid amplification techniques; optics; technology /technique development; toxicant screening; toxicology; toxin

DESCRIPTION (provided by applicant): The general aim of this proposal is to develop and commercialize an innovative technology for gene expression profiling. This proprietary technology is based on direct amplification of RNA by RNA polymerase. Efficient protocols will be established for amplification and detection of RNA, mRNAs in particular, that will be suitable for in field applications. This novel technology should provide great improvements in both efficiency and ease of use as compared to the methods currently available, for example, RT-PCR. With the recent advances in RNA sample extraction and preservation as well as optical signal detection, this technology will also enable highly sensitive and high-throughput assessment of changes to gene expression in response to environmental exposure. Feasibility of this system will be first demonstrated in the proposed studies. Products will then be developed to provide easy-to- use, inexpensive and expedient tools for the identification of perturbations of gene expression patterns in response to toxic exposure. Alternative protocols will be exploited in order to establish the optimum techniques. The methodology may be formatted for microarray. This system is expected to have wide potential use for environmental studies and epidemiology surveys, as we as in other fields of biomedical research and development. PROPOSED COMMERCIAL APPLICATION: Detection methods, detection kits, and components of detection system developed by others.

描述（由申请人提供）： 该提案的总体目标是开发一种创新的 基因表达谱分析技术。 这项专有技术是 基于RNA聚合酶直接扩增RNA。 有效的协议 将建立用于扩增和检测RNA，mRNA， 特别是将适用于现场应用。 这本小说 技术应该在效率和易用性两方面都提供很大的改进， 与目前可用的方法相比，例如RT-PCR。 与 RNA样品提取和保存的最新进展以及 光学信号检测，这项技术也将使高灵敏度 和高通量评估基因表达的变化， 环境暴露。 将首先论证该系统的可行性 在拟议的研究中。 然后将开发产品，以提供易于- 使用廉价和方便的工具来识别扰动 基因表达模式的变化。 替代 将利用各种协议，以确定最佳技术。 的 方法学可以被格式化用于微阵列。 预计该系统将具有 广泛的潜在用途的环境研究和流行病学调查，因为我们 就像在其他生物医学研究和开发领域一样。 拟定商业应用： 其他人开发的检测方法、检测试剂盒和检测系统组件。