SOLID STATE GENE MAPPING BY AFM

描述：(改编自申请人的摘要)。完成 对人类和其他基因组的了解是人类健康的核心 这个国家的公民。基因组分析中的一个关键步骤是确定 允许正确组装片段序列的物理图谱 信息转化为一个连续的基因组序列。这一阶段的目标是 SBIR的建议是测试高通量、固态的可行性 利用序列特异性物理标签和原子力显微镜绘制基因组图谱的方法。 大的靶DNA样本(例如Cosmid或YAC)被拴在定义的 表面上的地址。然后将它们与特定的序列孵化 地形标记，如催化折衷限制性内切酶 酵素。然后将样品梳理成线性陈列架，干燥并 由原子力显微镜检查。AFM快速生成样品的地形图， 用30个碱基对揭示标记在链上的位置 决议。这种方法的优点是速度快、经济和 最大限度地减少浪费。此外，序列标签的顺序是立即 已知的，这通常不是凝胶方法的情况。最后， AFM的独特属性将促进先进的未来应用， 基于分子力的基因分析和直接力学基因 操纵。 建议的商业应用：不可用

DESCRIPTION: (Adapted from the Applicant's Abstract). Complete understanding of the human and other genomes is central to the health of the nation's citizens. A key step in genome analysis is determination of a physical map that allows correct assembly of fragmented sequence information into a continuous genome sequence. The goal of this Phase I SBIR proposal is to test the feasibility of a high throughput, solid state genome mapping method using sequence specific physical tags and AFM. Large target DNA samples (e.g. cosmids or YACs) are tethered to defined addresses on a surface. They are then incubated with sequence specific topographical markers such as catalytically compromised restriction enzymes. The samples are then combed into a linear display, dried and examined by AFM. The AFM quickly generates a topograph of the sample, revealing the positions of the markers along the strand with 30 bp resolution. The advantages of this approach are speed, economy and minimal waste. Further, the order of the sequence tags is immediately known, which is usually not the case with gel-based methods. Finally, the unique attributes of the AFM will facilitate advanced future applications, molecular force-based gene analysis and direct mechanical gene manipulation. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE