XRAY ABSORPTION SPECT OF MANGANESE INVOLVED IN PHOTOSYNTHETIC OXYGEN EVOLUTION

我们正在调查涉及到的化学中间体 在高还原电势下转移电子并存储 生成O2所需的氧化当量。锰参与了一种 水氧化酶，我们正在使用x射线吸收 光谱分析以确定氧化状态、连接和局部 围绕着MN的结构。结合使用电子顺磁共振波谱的研究， 这些研究证实了锰氧化的直接参与。 水氧化过程中的状态变化。这项建议旨在 进一步表征四重态锰原子的结构 涉及的基本辅因子钙和氯化物、抑制剂和 通过使用X射线吸收光谱分析蛋白质配体。

We are investigating the chemical intermediates involved in transferring electrons at high reduction potentials and in storing the oxidizing equivalents needed to yield O2. Manganese is involved in the water-oxidizing enzyme, and we are using x-ray absorption spectroscopy to determine the oxidation states, ligation, and local structure around the Mn. Together with studies using EPR spectroscopy, these studies have verified the direct participation of Mn oxidation state changes in the water-oxidizing process. This proposal aims to characterize further the structure of the quartet of Mn atoms involved, the essential cofactors calcium and chloride, inhibitors and protein ligands through the use of X-ray absorption spectroscopy.