XRAY ABSORPTION SPECT OF MANGANESE INVOLVED IN PHOTOSYNTHETIC OXYGEN EVOLUTION

参与光合放氧的锰的 X 射线吸收谱

• 批准号: 6658666
• 负责人: MELVIN P KLEIN
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658666
• 关键词: biomaterials; biomedical resource; proteins; spectrometry; structural biology

We are investigating the chemical intermediates involved in transferring electrons at high reduction potentials and in storing the oxidizing equivalents needed to yield O2. Manganese is involved in the water-oxidizing enzyme, and we are using x-ray absorption spectroscopy to determine the oxidation states, ligation, and local structure around the Mn. Together with studies using EPR spectroscopy, these studies have verified the direct participation of Mn oxidation state changes in the water-oxidizing process. This proposal aims to characterize further the structure of the quartet of Mn atoms involved, the essential cofactors calcium and chloride, inhibitors and protein ligands through the use of X-ray absorption spectroscopy.

我们正在调查涉及的化学中间体， 在高还原电位下转移电子和在存储电子中， 产生O2所需的氧化当量。 锰参与了 水氧化酶，我们用X射线吸收 光谱法以确定氧化态、连接和局部 Mn周围的结构与使用EPR光谱的研究一起， 这些研究证实了Mn氧化的直接参与 水氧化过程中的状态变化。 这项建议旨在 进一步表征Mn原子四重态的结构 参与，必要的辅因子钙和氯，抑制剂和 蛋白质配体通过使用X射线吸收光谱。