HEAT LABILE ENTEROTOXIN FROM E COLI

来自大肠杆菌的不耐热肠毒素

• 批准号: 6437481
• 负责人: ETHAN A MERRITT
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437481
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

Synchrotron radiation is needed to collect higher resolution data from the LT-IIb holotoxin, both native crystals and soaked or co-crystallized with receptor analogues. If the MR structure solution has not succeeded by then we will use bromine-substituted sugars as receptor analogues, which should yield five Br sites for SIR/SAS phasing. We have constructed several active site mutants of LT, in addition to those previously crystallized. Experience to date is that these mutants produce small, radiation-sensitive, crystals which will require synchrotron radiation to produce adequate data quality.

需要同步辐射来收集更高分辨率的数据 从LT-IIb全毒素，无论是天然晶体和浸泡或 与受体类似物共结晶。 如果MR结构解决方案 到那时，我们将使用溴取代的糖作为 受体类似物，这应该产生5个溴位点的SIR/SAS 分阶段 我们构建了几个LT活性位点突变体， 除了那些以前结晶的。 迄今为止的经验是， 这些突变体产生小的、辐射敏感的晶体， 需要同步辐射来产生足够的数据质量。