Biomagnetic Signals of Intestinal Ischemia

描述(申请人提供)：肠缺血是否由 栓子、血栓形成或绞窄性梗阻仍然是一种严重和 有可能致命的情况。我们率先使用了超导技术 用于生物磁记录的量子干涉装置(SQUID)磁强计 体外、动物和人的胃肠平滑肌活性 研究对象。这种非侵入性、非接触式的生物磁流测量方法 Smooth Muscle论证了使用SQUIDs调查人类的可行性 肠道平滑肌生理学。鱿鱼有独特的基本优势 通过皮肤电极记录肠道电活动，因为 磁场很容易穿透脂肪层，而电场则不能 而人体小肠的皮肤电极记录不能常规 获得。鱿鱼代表了一种引人注目的新诊断设备，它没有 除了侵入性手术和将电极放置在 肠子表面。假设1：肠系膜缺血导致去偶联和 在外部记录中可检测到的肠平滑肌心律失常 磁场。我们将开发模型来解释心律失常、去偶联 以及介入性组织对外记录磁场的影响 菲尔兹。先进的分析技术区分缺血性肠病和正常肠病 信号将基于我们对平滑肌肉行为的知识而发展 在缺血期间。我们将第一次描述磁流的特征 在手术探查过程中和手术后的录音中。假设w： 肠系膜缺血导致受影响的平滑肌持续极化 导致在外部记录中可检测到的损伤电流的细胞 磁场。伤害电流以前已经被证明发生在 在心脏动物和人体研究中可以磁性地检测到这一点 现象。专门为研究人类而设计的新的多通道鱿鱼 作为SBIR赠款的一部分而开发和建造的肠道磁场 将可供使用，并将给我们带来非凡的 首次有机会探索血管平滑肌的病理生理学 肠缺血--人类已知的最致命的疾病之一。

DESCRIPTION (Provided by Applicant): Intestinal ischemia whether resulting from emboli, thrombosis or strangulation obstruction continues to be a serious and potentially fatal condition. We have pioneered the use of Superconducting Quantum Interference Device (SQUID) magnetometers for biomagnetic recordings of gastrointestinal smooth muscle activity in vitro, and in both animal and human subjects. This non-invasive, non-contact measurement of biomagnetic currents in smooth muscle demonstrates the feasibility of using SQUIDs to investigate human intestinal smooth muscle physiology. SQUIDs have unique fundamental advantages over cutaneous electrode recordings of intestinal electrical activity because magnetic fields readily penetrate layers of fat while electrical fields do not and cutaneous electrode recordings of human small bowel can not be routinely obtained. SQUIDs represent a striking new diagnostic device that has no equivalent except for invasive surgery and placement of electrodes onto the bowel surface. Hypothesis 1: Mesenteric ischemia causes uncoupling and arrhythmias of intestinal smooth muscle detectable in externally recorded magnetic fields. We will develop models to explain the arrhythmias, uncoupling and the effects of intervening tissue on the externally recording magnetic fields. Advanced analysis techniques to discriminate ischemic bowel from normal signals will be developed base upon our knowledge of smooth muscle behavior during ischemia. We will characterize for the fist time the magnetic currents in recordings taken during and after surgical exploration. Hypothesis w: Mesenteric ischemia causes sustained polarization of affected smooth muscle cells resulting in injury currents detectable in the externally recorded magnetic fields. Injury currents have been previously demonstrated to occur and be detectable magnetically in cardiac animal and human studies to detect this phenomenon. The new multichanel SQUID specifically designed for study of human intestinal magnetic fields developed and built as part of a SBIR grant will be available for use and will give us an extraordinary opportunity for the first time to explore smooth muscle pathophysiology during intestinal ischemia-one of the most deadly diseases known to man.