Biomagnetic Signals of Intestinal Ischemia

描述(申请人提供)：许多患者患有与小肠电活动障碍有关的疾病，然而，在人类受试者中研究这些问题的努力受到了阻碍，因为目前可用的电极技术需要外科植入，患者和他们的医生都不接受。近年来，生物磁成像方法首次促进了对受试者肠道慢波活动的无创评估。动物实验证实，经腹磁肠图(Meng)的磁场能准确反映肠道内慢波频率和空间频率梯度。我们已经建立并目前正在使用新的医院设施，范德比尔特大学胃肠鱿鱼技术(VU-GIST)实验室研究慢性肠系膜缺血对慢波的病理影响。我们准备继续为慢性肠系膜缺血患者的肠道慢波活动的生理和病理生理学意义做出开创性的贡献。我们建议通过继续开发记录和分析技术来推进这一点，这将使我们能够区分和区分来自小肠不同部分的生物磁场与其他物质来源(如结肠电活动)对生物磁场的多重贡献。作为慢性缺血的模型，我们将研究肠系膜血管部分近乎完全闭塞对Meng信号的影响。我们将对缺血肠进行更小的节段，以确定当前分析技术无创性识别局部缺血的能力，并为这种分析开发更先进的技术。我们将已经提供了令人信服的证据的初步动物研究反映了慢性肠系膜缺血患者手术前、术中和术后的病理慢波，我们将分析在这些研究中获得的生物磁场，以评估缺血和再灌注期间肠慢波的时空特征。该项目的最终目标是将SQUID发展成为肠系膜缺血患者的非侵入性诊断手段。

DESCRIPTION (provided by applicant): Many patients suffer from illnesses that relate to disturbances in the electrical activity of the small intestine, however efforts to investigate these problems in human subjects have been thwarted because currently available electrode technology require surgical implantation and are not accepted by patients nor their physicians. In recent years, biomagnetic imaging methods have, for the first time, facilitated the noninvasive assessment of the intestinal slow wave activity in human subjects. Animal studies have confirmed that transabdominal magnetic fields of the magnetoenterogram (MENG) accurately reflect the internal intestinal slow wave frequency and the spatial frequency gradient. We have established and are currently using new hospital facility, the Vanderbilt University Gastrointestinal SQUID Technology (VU-GIST) Laboratory study the pathological effects of chronic mesenteric ischemia on the slow wave. We are poised to continue our ground-breaking contributions to the physiological and pathophysiological implications of the activity the intestinal slow wave in patients with chronic mesenteric ischemia. We propose to advance these by continuing to develop the recording and analysis techniques that will allow us to differentiate and distinguish the multiple contributions to the biomagnetic field from different parts of the small intestine from other corporeal sources such as colonic electrical activity. We will examine the effect of partial near total occlusion of the mesenteric vessels on the MENG signal as a model of chronic ischemia. We will smaller segments of ischemic bowel to determine the ability of current analysis techniques to identify localized ischemia noninvasively and to develop more advanced techniques for this analysis. We will our initial animal studies that have provided convincing evidence that the MENG reflects the underlying pathological slow wave to pre-, intra- and post-operative studies of human patients with chronic mesenteric ischemia, and we will analyze the biomagnetic fields obtained in these studies to assess the spatiotemporal signatures of intestinal slow waves during ischemia and reperfusion. The ultimate goal of this project is to develop the SQUID into a noninvasive diagnostic modality for patients with mesenteric ischemia.