STRUCTURE & FUNCTION OF BOMBESIN LIKE PEPTIDES

结构

• 批准号: 6453684
• 负责人: ELIOT R SPINDEL
• 金额: $ 11.11万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6453684
• 关键词: Chordata; Mammalia; biotechnology; endocrine gland /system; growth /development; inborn metabolism disorder; mother /infant health care; nervous system; respiratory system

To date, two mammalian bombesin-like peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB), have been characterized. These two peptides are widely distributed in mammalian nervous system and GI tract; but, of greatest clinical importance, GRP is expressed by many neoplasms and may stimulate the growth of these neoplasms. Recent studies suggest that there are a number of additional bombesin-like peptides that are yet to be identified and that these peptides likely play an important role in growth and development. Thus, we are attempting to identify new bombesin-like peptides by cross-hybridization with cDNAs encoding previously characterized bombesin-like peptides, as well as by using antibodies to amphibian bombesin-like peptides. Once new bombesin-like peptides are discovered, distribution studies and physiologic studies using the primate model will be established to determine the role of these new peptides in normal and neoplastic processes. To date , we have discovered 3 new amphibian bombesin-like peptides and two new amphibian bombesin receptors. Our new data suggests that the ligand for recently described bombesin BRS-3 receptor may turn out to be the long sought mammalian bombesin. FUNDING NIH CA39237 PUBLICATIONS Pradhan TK, Katsuno T, Ryan RR, Mantey SA, Akeson MA, Donohue PJ, Battey JF, Spindel ER. The bombesin receptor subtype 4 is coupled to phospholipase C and has a unique pharmacology. In Meeting of the American Gastroenterology Society (held in New Orleans, LA, May 16-20, 1998 (abstract).

迄今为止，两种哺乳动物蛙皮素样肽，胃泌素释放肽， 肽（GRP）和神经介肽B（NMB）。 这些 这两种肽广泛分布于哺乳动物的神经系统和胃肠道， 道;但是，最重要的临床意义，GRP是由许多表达 肿瘤，并可能刺激这些肿瘤的生长。 最近 研究表明，有一些额外的蛙皮素样 这些肽还没有被鉴定出来， 在增长和发展中发挥重要作用。 因此，我们 试图通过以下方法鉴定新的蛙皮素样肽： 与编码先前表征的cDNA的交叉杂交 蛙皮素样肽，以及通过使用两栖动物抗体， 蛙皮素样肽。 一旦新的蛙皮素样肽 发现，分布研究和生理学研究，使用 将建立灵长类动物模型，以确定这些新的 肽在正常和肿瘤过程。 到目前为止， 发现了3种新的两栖动物蛙皮素样肽和两种新的 两栖类蛙皮素受体 我们的新数据表明 最近描述的蛙皮素BRS-3受体可能是 一直在寻找哺乳动物蛙皮素 资助NIH CA 39237出版物 Pradhan TK，Katsuno T，Ryan RR，Mantey SA，Akeson MA，Donohue PJ， Battey JF Spindel ER. 蛙皮素受体亚型4与 磷脂酶C具有独特的药理作用。 在会议上， 美国胃肠病学学会（5月16日至20日在路易斯安那州新奥尔良举行， 1998年（摘要）。