MOLECULAR AND CELLULAR BIOLOGY CORE

分子和细胞生物学核心

• 批准号: 8125612
• 负责人: ELIOT R SPINDEL
• 金额: $ 6.87万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8125612
• 关键词: Age; Antibiotics; Automation; Bacteriophages; Blood capillaries; Cell Culture Techniques; Cell Line; Cellular biology; Clone Cells; Cloning; Culture Media; DNA Sequence; Deposition; Equipment; Expressed Sequence Tags; Genbank; Generations; Goals; Grant; Hormones; Hypothalamic structure; In Situ Hybridization; Large-Scale Sequencing; Libraries; Liquid substance; Macaca mulatta; Molecular Biology; Molecular and Cellular Biology; Monkeys; National Institute of Child Health and Human Development; Nicotinic Receptors; Ovary; Plasmids; Preparation; Primates; Protocols documentation; RNA; Reaction; Reader; Reading; Reagent; Research Personnel; Resources; Ribonucleases; Robot; Running; Services; Taq Polymerase; Techniques; Time; Training; Transfection; United States National Institutes of Health; cDNA Library; capillary; cost effective; culture plates; design; flexibility; improved; instrumentation; member; microbial; serotonin receptor; tissue culture

The Molecular and Cellular Biology (MCB) core will provide services in support of molecular biology and cell culture for U54 investigators and scientific staff at the ONPRC with NICHDsupported projects. The goal of the MCB Core is to provide flexible, time- and cost-effective services to facilitate investigator's utilization of molecular biology and cell culture. Molecular biology services include generation of monkey specific cDNAs; automated sequencing; large scale plasmid and phage preparation; supply of a limited number of reagents, training and access to specialized equipment including a liquid-handling robot, realtime PCR and phosphorimaging. Cell biology services include preparation of media and reagents, storage and amplification of cell lines, preparation of coated culture ware and cover slips, transfection and cloning of cell lines, training, and access to specialized equipment including a fluorescent plate reader. The MCB Core will be an open core dedicating 45% of core resources to 3 of the U54 projects and the remaining resources to 7 U54-approved ROls

分子和细胞生物学（MCB）核心将在nichd支持的项目中为ONPRC的U54研究人员和科学人员提供分子生物学和细胞培养支持服务。MCB核心的目标是提供灵活，时间和成本效益的服务，以促进研究者利用分子生物学和细胞培养。分子生物学服务包括猴特异性cdna的生成；自动测序;大规模质粒和噬菌体制备；供应数量有限的试剂，培训和获取