Molecular and Cellular Biology Core

分子和细胞生物学(MCB)核心将通过NICHD支持的项目为ONPRC的U54研究人员和科学人员提供支持分子生物学和细胞培养的服务。MCB核心的目标是提供灵活、节省时间和成本效益的服务，以促进研究人员利用分子生物学和细胞培养。分子生物学服务包括产生猴子特有的cDNA；自动测序；大规模的质粒和噬菌体制备；提供有限数量的试剂，培训和获得 专门的设备包括液体处理机器人、实时聚合酶链式反应和磷成像。细胞生物学服务包括准备培养基和试剂，储存和放大细胞系，准备涂布的培养器皿和盖片，细胞系的转基因和克隆，培训，以及获得包括荧光平板阅读器在内的专门设备。MCB核心将是一个 开放核心，将45%的核心资源用于U54项目中的3个，其余资源用于7个U54核准的ROL

The Molecular and Cellular Biology (MCB) core will provide services in support of molecular biology and cell culture for U54 investigators and scientific staff at the ONPRC with NICHDsupported projects. The goal of the MCB Core is to provide flexible, time- and cost-effective services to facilitate investigator's utilization of molecular biology and cell culture. Molecular biology services include generation of monkey specific cDNAs; automated sequencing; large scale plasmid and phage preparation; supply of a limited number of reagents, training and access to specialized equipment including a liquid-handling robot, realtime PCR and phosphorimaging. Cell biology services include preparation of media and reagents, storage and amplification of cell lines, preparation of coated culture ware and cover slips, transfection and cloning of cell lines, training, and access to specialized equipment including a fluorescent plate reader. The MCB Core will be an open core dedicating 45% of core resources to 3 of the U54 projects and the remaining resources to 7 U54-approved ROls