Molecular and Cellular Biology Core

分子和细胞生物学核心

基本信息

批准号：
7788356
负责人：
ELIOT R SPINDEL
金额：
$ 6.87万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-01 至 2011-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7788356
关键词：
Age Antibiotics Automation Bacteriophages Blood capillaries Cell Culture Techniques Cell Line Cellular biology Clone Cells Cloning Culture Media DNA Sequence Deposition Equipment Expressed Sequence Tags Genbank Generations Goals Grant Hormones Hypothalamic structure In Situ Hybridization Infertility Large-Scale Sequencing Libraries Liquid substance Macaca mulatta Molecular Biology Molecular and Cellular Biology Monkeys National Institute of Child Health and Human Development Nicotinic Receptors Ovary Plasmids Preparation Primates Protocols documentation RNA Reaction Reader Reading Reagent Research Research Personnel Resources Ribonucleases Robot Running Services Taq Polymerase Techniques Time Training Transfection United States National Institutes of Health cDNA Library capillary cost culture plates design flexibility improved instrumentation member microbial serotonin receptor tissue culture

项目摘要

The Molecular and Cellular Biology (MCB) core will provide services in support of molecular biology and cell culture for U54 investigators and scientific staff at the ONPRC with NICHDsupported projects. The goal of the MCB Core is to provide flexible, time- and cost-effective services to facilitate investigator's utilization of molecular biology and cell culture. Molecular biology services include generation of monkey specific cDNAs; automated sequencing; large scale plasmid and phage preparation; supply of a limited number of reagents, training and access to specialized equipment including a liquid-handling robot, realtime PCR and phosphorimaging. Cell biology services include preparation of media and reagents, storage and amplification of cell lines, preparation of coated culture ware and cover slips, transfection and cloning of cell lines, training, and access to specialized equipment including a fluorescent plate reader. The MCB Core will be an open core dedicating 45% of core resources to 3 of the U54 projects and the remaining resources to 7 U54-approved ROls

分子和细胞生物学（MCB）核心将为ONPRC的U54研究人员和科学人员提供Nichdsporport的项目的分子生物学和细胞培养的服务。 MCB核心的目的是提供灵活，时间和成本效益的服务，以促进研究者对分子生物学和细胞培养的利用。分子生物学服务包括生成猴子特定的cDNA；自动测序；大规模的质粒和噬菌体制剂；供应有限数量的试剂，培训和进入专用设备，包括液体处理机器人，实时PCR和磷光成像。细胞生物学服务包括培养基和试剂的制备，细胞系的存储和扩增，涂层培养厂和盖上覆盖滑动的准备，转染和克隆的细胞系，训练以及对包括荧光板读取器在内的专用设备的访问。 MCB核心将是一个开放核心将45％的核心资源专用于3个U54项目，其余的资源向7个U54批准的ROLS