Discovery of natural products that are critical for controlling plant pathogens (TRUMAN_J17DTP)

发现对控制植物病原体至关重要的天然产物 (TRUMAN_J17DTP)

• 批准号: 1937478
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1937478
• 关键词: Discovery; natural; products; critical; controlling

Plant diseases are a huge problem for many crops, and can cause a vast amount of ecological and economic damage. In many cases, it is difficult to control these diseases as current treatments are ineffective, expensive or environmentally damaging. A promising route to disease control is the use of bacterial biocontrol strains that naturally inhibit pathogen growth, and there is significant evidence to show that bacterial natural products are critical for the suppression of various pathogens. This is consistent with the crucial role that natural products have across medicine (e.g. antibiotics) and agriculture (e.g. insecticides).This project will investigate a novel family of Pseudomonas natural products that were discovered using a pathogen suppression screen using common scab, an economically important bacterial disease that affects potatoes. These compounds will be structurally characterised, and their bioactivities, regulation and biosynthesis will be determined. The project will also employ our genome-based screening approach to identify natural products that contribute to the suppression of other plant diseases, with the ultimate aim of developing compounds or strains that can be used to control plant disease.

植物病害对许多作物来说是一个巨大的问题，并可能造成大量的生态和经济损失。在许多情况下，很难控制这些疾病，因为目前的治疗方法是无效的，昂贵的或破坏环境的。一种有希望的疾病控制途径是使用天然抑制病原体生长的细菌生物防治菌株，并且有大量证据表明细菌天然产物对于抑制各种病原体至关重要。这与天然产物在医药（如抗生素）和农业（如杀虫剂）中的重要作用是一致的。本项目将研究一个新的假单胞菌天然产物家族，该家族是使用普通疮痂病（一种影响马铃薯的经济上重要的细菌性疾病）进行病原体抑制筛选而发现的。这些化合物将在结构上进行表征，并确定其生物活性，调节和生物合成。该项目还将采用我们基于基因组的筛选方法来识别有助于抑制其他植物疾病的天然产物，最终目的是开发可用于控制植物疾病的化合物或菌株。

项目成果

A User Guide for the Identification of New RiPP Biosynthetic Gene Clusters Using a RiPPER-Based Workflow.

使用基于 RiPPER 的工作流程鉴定新 RiPP 生物合成基因簇的用户指南。

• DOI: 10.1007/978-1-0716-1358-0_14
• 发表时间: 2021
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Moffat AD

A biofoundry workflow for the identification of genetic determinants of microbial growth inhibition.

• DOI: 10.1093/synbio/ysab004
• 发表时间: 2021
• 期刊: Synthetic biology (Oxford, England)
• 作者: Moffat AD;Elliston A;Patron NJ;Truman AW;Carrasco Lopez JA
• 通讯作者: Carrasco Lopez JA