Instrumentation And Bioengineering Development

在许多情况下，使用新方法开发独特的仪器是生物医学工程成功的必要因素。1)激光闪光光解与血红蛋白结合的配体可以评估动力学结合常数，用于了解血红蛋白的作用机制。电子仪器已经发展到控制亚微秒测序和实验程序的操作，并捕获监测反应动力学的光信号。2)在开颅患者中进行实时功能性红外成像的能力，可以通过非侵入性方法可靠地定位病变。将红外图像视频帧与刺激、ECG或脑放电同步，使信号强度变化与生理参数相关，如能够识别肿瘤病变的灌注。用于生产视网膜电图非标准刺激的电子和机电系统的发展使得诊断测试和研究方案取得了进展。4)同时收集光学和化学组分的机械和光学设计增强了通过超离心分析建立的大分子沉降平衡的实用性。5)测量各种仪器的稳态和动态性能特性，用于心血管研究。6)荧光光谱法已发展用于表征神经递质调节。

Development of unique instrumentation often using novel approaches is, in many instances, a necessary attribute to the success of biomedical engineering. 1) Laser flash photolysis of ligands bound to hemeproteins enables kinetic binding constants to be evaluated for use in understanding the mechanism of action of hemeproteins. Electronic instrumentation has been developed to control the sub-microsecond sequencing and operation of experimental procedures and to capture optical signals monitoring the reaction kinetics. 2) The ability to perform real time functional infra-red imaging in patients undergoing craniotomy permits reliable localization of lesions with a non-invasive approach. Synchronization of infra-red image video frames to stimuli, ECG, or brain electrical discharge allows signal intensity changes to be correlated with physiological parameters such as perfusion that enable tumor lesion identification. 3) Development of electronic and electro-mechanical systems for production of non-standard stimuli for electroretinography has allowed advances in diagnostic testing and research protocols. 4) Mechanical and optical designs to gather simultaneous optical and chemical fractions enhances the utility of macromolecular sedimentation equilibria established by ultracentrifugation analysis. 5) Measuring steady state and dynamic performance characteristics of various instruments is utilized for cardiovascular research. 6) Spectrofluorometric methods have been developed for characterization of neurotransmitter regulation.